background: streptokinase is a potent activator of plasminogen to plasmin, the enzyme that can solubilize the fibrin network in blood clots. streptokinase is currently used in clinical medicine as a thrombolytic agent. it is naturally secreted by β-hemolytic streptococci. methods: to reach an efficient method of purification, an immunoaffinity chromatography method was developed that could puri...

background: botulinum neurotoxin (bont) complexes consist of neurotoxin and neurotoxin-associated proteins. hemagglutinin-33 (ha-33) is a member of bont type a (bont/a) complex. considering the protective role of ha-33 in preservation of bont/a in gastrointestinal harsh conditions and also its adjuvant role, recombinant production of this protein is favorable. thus in this study, ha-33 was expr...

background and objective: use of elastin-like proteins (elps) provides high-performance protein purification without need for chromatography. in line with cost reduction and facilitation of recombinant proteins purification, which represent a high percentage of production costs, in this project, we eliminated the need for proteases in the process of separation of recombinant proteins from elp b...

Once a protein purification scheme has been developed, the purification, characterization, and use/structure of a target protein are usually published. It is highly desirable to present the major steps in the purification and the corresponding features of the protein at each step summarized in the form of a purification summary table. In considering whether to repeat a published protein purific...

The toxicity and corrosion potential of hydrogen sulfide in raw biogas underlines the need for biogas purification. Several techniques available for removal of hydrogen sulfide from biogas are out of the reach for common end users due to lack of knowledge, higher running costs, and insufficient operational skills. The present experimental study aims to propagate hydrogen sulfide removal techniq...

background: the presence of lead in natural waters has become an important issue around the world. lead has been identified as a highly toxic metal that can cause severe environmental and public health problems and its decontamination is of utmost importance. the aim of this work was to evaluate the adsorption of lead (pb(ii)) on waste tea leaves as a cheap purification method. methods: in this...

background and objectives: recognizing the impurities of sugar beet and also comparing different methods of juice purification have shown that the chemical purification based on lime and carbon dioxide is really efficient and economic in the sugar industry. factories have to control and improve purification stages to achieve clear juice with high chemical purity, low color, minimum hardness, su...

introduction: polyclonal antibodies are required to be affinity purified. improved purification methods of polyclonal antibody provide an opportunity to pick the most purified immunoglobulins as a primary or secondary antibody in immunoassays that are included in many vaccine studies. two common techniques for purifying proteins is salt precipitation and chromatography purification. our work fo...

one of the most important aspects in recombinant biologic production, based on gmp rules, is the accuracy of final product quality control, especially assessment of host cell macromolecules contamination rate in final product. the purification requirement can be eliminated when the yeast cell containing the recombinant protein is used as a host cell. it is possibile that the final product conta...