Silymarin is having antioxidant and membrane stabilizing property and acts as a defensive material in different toxic forms of liver diseases like liver cirrhosis, alcoholic liver disease. Pullulan is a good water soluble neutral linear polysaccharide consisting of α-1,6 linked maltotriose residues. It is broadly used as a food additive and is considered to be safe for human use. In the present...

The production of pullulan from molasses and synthetic medium by Aureobasidium pullulans P56 in batch culture was investigated. Among the pretreatments of molasses used to increase polysaccharide production, sulfuric acid + activated carbon treatment gave better results with regard to polysaccharide concentration, polysaccharide yield and sugar utilization. A maximum polysaccharide concentratio...

The present study discusses evaluation of pullulan-functionalized doxorubicin nanoparticles for asialoglycoprotein receptor-mediated uptake in the Hep G2 cell line. Doxorubicin hydrochloride (DOX) nanoparticles using polymers of different hydrophobic character, polyethylene sebacate (hydrophobic) and poly (lactic-co-glycolic acid) (intermediate hydrophobicity) with high entrapment efficiency an...

BACKGROUND poly(L-malic acid) (PMA) is a water-soluble polyester with many attractive properties in medicine and food industries, but the high cost of PMA fermentation has restricted its further application for large-scale production. To overcome this problem, PMA production from Jerusalem artichoke tubers was successfully performed. Additionally, a valuable exopolysaccharide, pullulan, was co-...

The commercially available pullulan (Pullulan PI-20) has a purity of more than 90 %. Its average molecular weight (at peak of a gel permeation chromatogram) is about 200 kD. The main impurities are mono-, diand oligosaccharides, which are carried over from the raw material (hydrolysed starch) into the final product. The specifications for Pullulan include standard parameters for identification ...

Fruits form an important part of a healthy human diet as they contain many ingredients with proven pro-health effects such as vitamins, phenolic compounds, organic acids, fiber, and minerals. The purpose of this work was to evaluate the effect of pullulan coating on the quality and shelf life of highbush blueberry during storage. General appearance, weight loss, dry matter, soluble solid conten...

Downstream processing is an important aspect of all biotechnological processes and has significant implications on quality and yield of the final product. Several solvents were examined for their efficacy on pullulan precipitation from fermentation broth. Interactions among four selected solvents and their effect on pullulan yield were studied using response surface methodology. A polynomial mo...

We quantify the hydrodynamic screening length (n) in j-carrageenan solutions by observing restrictions on the diffusion of several pullulan probes, each with a different molecular weight (Mw). The diffusion coefficient of pullulan in a pure solvent (Dpull,0) and a j-carrageenan solution (Dpull) were measured using a pulsed field gradient stimulated spin-echo 1H nuclear magnetic resonance. The h...

The anion exchanger phosphocellulose and the cation exchanger triethylaminoethyl cellulose were used to immobilize cells of the fungus Aureobasidium pullulans ATCC 201253 and the adsorbed cells were subsequently investigated for their ability to produce the polysaccharide pullulan using batch fermentation. The cells adsorbed on the triethylaminoethyl cellulose at pH 7.5 produced higher pullulan...

Total 95 isolates of Aureobasidium pullulans were isolated from different flowers and leaves samples, out of which 11 thermotolerant strains produced pullulan. One thermotolerant non-melanin pullulan producing strain, designated as RG-5, produced highest pullulan (37.1±1.0 g/l) at 42(o)C, pH 5.5 in 48h of incubation with 3% sucrose and 0.5% ammonium sulphate in a non-stirred fed batch fermentor...