conclusions the results of the present study will increase our knowledge about molecular cloning and expression of the l. infantum kmp-11 gene, and this may be used as an effective target for controlling visceral leishmaniasis. results the kmp-11 gene was successfully subcloned in pcdna3 as a eukaryotic expression vector. recombinant kmp-11 protein was confirmed by the reverse transcriptase pol...

DNA fingerprints of four rose species, Rosa centifolia, R. Gruss-an-Teplitz, R. bourboniana, and R. damascena, were developed using RAPD-PCR. We identified a unique polymorphic band in R. centifolia. This 762-bp fragment was produced by the random primer GLI-2. The fragment was eluted and directly cloned in a TA cloning vector, pTZ57R/T. Digestion of the plasmid with EcoRI confirmed the c...

objective: leishmaniasis is caused by parasitic protozoa of the genus leishmania which in the infected host is obligate intracellular parasite. lack gene is conserved among related leishmania species. lack is the immuno-dominant antigen of l.major which is considered as the most promising molecule for a recombinant or dna vaccine against leishmaniasis. materials and methods: in this study the ...

dna amplification using taq dna polymerase is one of the most widely used techniques in molecular biology and biotechnology. the aim of this study was to amplify the gene of this enzyme from a thermophilic bacteria called thermus aqauticus and clone it into a vector for future use. using specific primers the cdna of taq dna polymerase was amplified and ligated into the cloning vector ptz57r usi...

genetic typing methods of t. gondii strains have been extensively perfected in recent years. from a technical point of view, many tools usable for genetic studied on single-copy loci have been used: rflp, pcr-rflp, sequencing, rapd-pcr and isoenzyme analysis. we described the cloning and sequence analysis of the gene which encodes the major surface antigen (sag1 or p30) of t. gondii. sag1 is th...

due to the widespread applications of xylitol dehydrogenase, an enzyme used for the production of xylitol, the present study was designed for the cloning of xylitol dehydrogenase gene from glcunobacter oxydans dsm 2003. after extraction of genomic dna from this bacterium, xylitol dehydrogenase gene was replicated using polymerase chain reaction (pcr). the amplified product was entered into ptz5...

background brucellosis is an important cosmopolitan infection disease caused by organisms belonging to the genus brucella. the cgt gene (cyclic β-1, 2 glucan transporter gene) is a virulent factor in brucella genus. the present study was conducted with the aim of cloning and expression of brucella cgt gene. materials and methods brucella melitensis cgt gene was amplified from extracted chromoso...

conclusions the results of this study demonstrated that the p450 cyp141 gene was successfully cloned into a pet26b plasmid vector as an expression vector. in this paper, for the first time in iran, this gene was cloned for more purposes, including the expression and purification of the recombinant cytochrome p450 cyp141 protein. results the cloning of p450 cyp141 gene was confirmed by the enzym...

Fibrinolytic therapy progressed by the evolution of different strategies that helped in enhancing its efficacy and specificity. The use microbial fibrinolytic enzymes is leading to a promising direction for treatment cardiovascular diseases. Subtilisin, member subtilases enzyme abundantly found Bacillus species. isolation subtilisin gene (quaking homolog) from subtilis was attempted present wor...