the synthetic seed technology is becoming popular due to its wide application in germplasm conservation and for exchanges between countries in the floriculture trade. in this study, this method was used to study the germination and conversion capabilities of orchid species dendrobium white fairy when stored at different storage times and with different storage containers. a high germination per...

Dendrobium officinale Kimu et Migo has increased many researchers' interest for its high medical and horticultural values and the molecular mechanism of its protocorm development remains unclear. In this study, 19 genes from 26 most stably expressed genes in whole transcriptome of protocorms and 5 housekeeping genes were used as candidate reference genes and screened with 4 application software...

Protocorm pieces of the orchid Cymbidium were aseptically cultured either without phytohormones, or with one of the growth promoting substances, auxin cytokinin, and gibberellin. The derivative melting profiles of the extracted DNA's differ from each other with respect to the size of various AT- and GC- rich fractions. Evidence has been obtained for the increase of the more AT--rich fractions i...

The Dendrobium Orchid Gatton Sunray is a hybrid orchid of pulchellum × illustre . A study was aimed to find the best type medium and banana for multiplication Protocorm-Like Bodies (PLBs) this orchid. This research conducted from February September 2020 at Plant Tissue Culture Laboratory, Faculty Agriculture, University Bengkulu. Treatments included media cultivar ripe fruit, added as source or...

Protocorm của cây lan Hoàng thảo Giả hạc (Dendrobium anosmum Lindl.) bốn tuần tuổi được sử dụng để làm vật liệu khởi đầu cho nhân giống in vitro. Dịch chiết gốc vi khuẩn lam Arthrospira sp. tạo thành bằng cách nghiền 1 g sinh khối tươi trong 100 mL nước cất. Kết quả thấy dịch có tác tăng cường sự chồi và ra rễ nuôi cấy Môi trường MS cơ bản bổ sung 6 g·L–1 agar, 30 saccharose 1,5 mg·L–1 BAP kết ...

BACKGROUND AND AIMS Cymbidium mastersii is an epiphytic orchid distributed mainly in Northeast India. Owing to its high commercial value in the floricultural industry, natural populations are under threat from over-exploitation. Mass propagation provides an alternative means of satisfying the demand. Unfortunately, conventional propagation is slow and difficult, suggesting in vitro methods for ...

Two prevalent issues with Gastrodia elata growing in a natural wild environment are the necessary symbiotic seed germination Mycena spp. and long cycle of protocorms into mature rhizomes Armillaria mellea. This study explored most suitable nutrients to germinate seeds developmental stages propagated protocorm/juvenile (jrhs) through tissue culture approach. Adding humic acid, mashed potato, pep...

BACKGROUND AND AIMS Dendrobium longicornu, commonly known as the 'Long-horned Dendrobium', is an endangered and medicinally important epiphytic orchid. Over-exploitation and habitat destruction seriously threaten this orchid in Northeast India. Our objective was to develop an efficient protocol for the mass propagation of D. longicornu using axillary bud segments. METHODOLOGY AND PRINCIPAL RE...

In vitro asymbiotic seed germination of Dendrobium chrysotoxum varied with fruit harvesting time and culture medium used for germinating seeds. Seeds at different developmental stages were harvested and cultured on five asymbiotic orchid seed germination media i.e. defined and undefined media, namely 1⁄2 MS, KC, M, MS and PDA. Seeds harvested 220 days after pollination showed maximum germinatio...