Background: The accumulation of ubiquitinated proteins has been detected in diseased hearts and associated with the expression p62 microtubule-associated protein 1 light chain 3 (LC3), which are related to autophagy. We evaluated differences ubiquitin LC3 cardiomyopathy using endomyocardial biopsies.

Abstract The cytoskeleton is a major focus of physical studies to understand organization inside cells given its primary role in cell motility, division, and mechanics. Recently, protein condensation has been shown be another intracellular organizational strategy. Here, we report that the microtubule crosslinking proteins, MAP65-1 PRC1, can form phase separated condensates at physiological salt...

•SBTub3 photocontrols microtubule dynamics, organization, and dependent processes •Microtubule photocontrol is cell sub-cellularly precise temporally reversible •SBT orthogonal to GFP/YFP imaging SBTs are metabolically stable •The SBT scaffold promising for photopharmaceuticals other protein targets Optically controlled chemical reagents, termed “photopharmaceuticals,” powerful tools spatiotemp...

Background: In this study, we examined a new two step induction protocol for improving the differentiation of human umbilical cord blood-derived mesenchymal stem cells into neural progenitor cells. Materials and Methods: Human umbilical cord blood-derived mesenchymal stem cells were first cultured in Dulbecco’s modified eagle medium supplemented with 10% fetal bovine serum in a humidified incu...

Post-translational modifications of proteins are ubiquitous in living organisms, as they enable an accurate control the interactions these macromolecules. For mechanistic studies, it would be highly advantageous to able produce vitro post-translationally modified with site-specificity. Here, we demonstrate one facile way achieve this goal through use post-translational chemical mutagenesis. We ...

background: in this study, we examined a new two step induction protocol for improving the differentiation of human umbilical cord blood-derived mesenchymal stem cells into neural progenitor cells. materials and methods: human umbilical cord blood-derived mesenchymal stem cells were first cultured in dulbecco’s modified eagle medium supplemented with 10% fetal bovine serum in a humidified incub...