prolactin is mainly secreted by the anterior pituitary and is able to stimulate mammary gland development and lactation in mammalians. although prolactins share a common ancestral gene encoding, they show species specific characteristics and their efficiency may be different in various mammals. the importance of protein structures of all sequences of this hormone have been studied by various bi...

a number of biological processes in vertebrates, including reproduction, growth and development, osmoregulation, metabolism and immunoregulation (Nicoll, 1974). The biological actions of prolactin have been most extensively characterized in the mammary gland, where it has an essential role both in the differentiation of the gland during pregnancy and in the regulation of milk protein gene expre...

The prolactin family represents a group of hormones and cytokines that participate in the control of maternal and fetal adaptations to pregnancy. The aim of this study was to develop a simple assay for monitoring patterns of prolactin family gene expression in rats and mice. Prolactin family cDNAs were spotted on to nylon membranes. Total RNA was extracted from tissues or cells. cDNAs were gene...

Prolactin is mainly secreted by the anterior pituitary and is able to stimulate mammary gland development and lactation in mammalians. Although prolactins share a common ancestral gene encoding, they show species specific characteristics and their efficiency may be different in various mammals. The importance of protein structures of all sequences of this hormone have been studied by various bi...

We previously reported that prolactin gene expression in the T-leukemic cell line Jurkat is stimulated by PGE(2) and that cAMP acts synergistically with Ca(2+) or protein kinase C on the activation of the upstream prolactin promoter. Using the transcription inhibitor actinomycin D, we now show that PGE(2)-induced prolactin expression requires de novo prolactin mRNA synthesis and that PGE(2) doe...

Prolactin has many different biological functions. It stimulates Nb2 cell multiplication through the kinase C transduction mechanism, casein secretion through the phospholipase A2-arachidonic acid-leukotrienes cascade and milk protein gene expression through an unknown mechanism. Colchicine and other tubulin binding drugs inhibit casein gene expression and DNA synthesis stimulated by prolactin ...

During lactation, adult female mice display aggressive responses toward male intruders, triggered by male-derived chemosensory signals. This behavior is not shown pup-sensitized virgin females sharing pup care with dams. The genetic mechanisms underlying the switch from attraction to aggression are unknown. In this work, we investigate differential gene expression in lactating expressing matern...

Abbreviations: ICW – In-Cell Western assay, MAP2 – microtubule-associated protein 2, PBS – phosphate-buffered saline, RT – room temperature. Abstract. Although many studies have demonstrated the role of prolactin in the central nervous system, there is a considerable lack of known effects of prolactin on the parameters of neurogenesis and neuronal differentiation. The aim of the present study w...

Polymorphism. A HaeIII PCR-RFLP was identified in the ovine prolactin ( PRL) gene. Source and Description of Primers. Human genomic (Truong et al., 1984) and pig cDNA (GenBank accession no. X14068) sequences were compared to design primers to span the second intron of the prolactin gene.

A fusion gene containing 5' rat prolactin genomic sequences ligated to the structural portion of the rat growth hormone gene ( grl ) was introduced by DNA-mediated gene transfer into mammalian cells by using a chimeric plasmid vector. Clonal transfected cell lines produced a mRNA that used the authentic 5' initiation site and that was processed to the predicted size. The intracellular levels of...