background: potyviruses are accounted for 40% of viral diseases of various crops including vegetables and legumes. potyviruses can be transmitted through plant sap, seeds and many aphid species. due to ease of spread of these viruses detection of such viruses are crucial as to the control of the incited diseases. objectives: this study compared the efficiencies of two couples of primers in thei...

Cellular RNA-dependent RNA polymerases (RDRs) catalyze synthesis of double-stranded RNAs that can serve to initiate or amplify RNA silencing. Arabidopsis thaliana has six RDR genes; RDRs 1, 2 and 6 have roles in anti-viral RNA silencing. RDR6 is constitutively expressed but RDR1 expression is elevated following plant treatment with defensive phytohormones. RDR1 also contributes to basal virus r...

A new carlavirus, tentatively named Potato virus H (PVH), was found on potato plants with mild symptoms in Hohhot, Inner Mongolia Autonomous Region, China. PVH was confirmed by genome sequencing, serological reactions, electron microscopy, and host index assays. The PVH particles were filamentous and slightly curved, with a modal length of 570 nm. Complete RNA genomic sequences of two isolates ...

the use of healthy planting material is an important prerequisite for controlling destructive viral diseases in potato production. virus elimination methods such as meristem culture and thermotherapy are usually used to produce the nucleus stock of healthy planting material. here, we report a new technique of electrotherapy for elimination of two potyviruses, potato virus a (pva), and potato vi...

Chromosome pairing relationships within cultivated potato (Solanum tuberosum) and its wild tuber-bearing relatives (Solanum sect. Petota) have been interpreted by genome formulas, developed in the early 1900s, through techniques of classic meiotic analysis of interspecifi c hybrids. Here we reexamine potato genome hypotheses with the fi rst phylogenetic analysis of all major genomes of sect. Pe...

Potato leafroll virus (PLRV; genus Polerovirus , family Luteoviridae) is a persistently transmitted circulative virus that depends on aphids for spreading. The primary vector of PLRV is the aphid Myzus persicae (Sulzer) (Homoptera: Aphididae). Solanum tuberosum L. potato cv. Kardal (Solanaceae) has a certain degree of resistance to M. persicae : young leaves seem to be resistant, whereas senesc...

A nationwide survey was performed to investigate the current incidence of viral diseases in Korean sweet potatoes for germplasm and growing fields from 2011 to 2014. A total of 83.8% of the germplasm in Korea was infected with viruses in 2011. Commercial cultivars that were used to supply growing fields were infected at a rate of 62.1% in 2012. Among surveyed viruses, the incidence of five Poty...

Potato virus Y (PVY) and Potato mop-top virus (PMTV) are viruses whose geographical distribution is expanding and economic losses are increasing, in contrast to most of other viruses infecting potato crops. Most potato cultivars lack broad-spectrum resistance to the new, genetically complex strains of PVY, and no efficient resistance to PMTV is known in potato. Control of the vectors of these v...

A full-length cDNA copy of the genome of Potato leafroll virus (PLRV) was introduced into the genome of tobacco and potato plants by Agrobacterium tumefaciens-mediated transformation. Transgenic lines were obtained in which the transgene was readily detected by PCR with DNA extracted from T(1) tobacco seedlings and clonally multiplied potato plants. PLRV-specific genomic and sub- genomic RNAs, ...

Eight viruses infect ulluco (Ullucus tuberosus Caldus), an Andean tuber crop. Three of them, potato leafroll virus (PLRV), Andean potato latent virus, and potato virus T, also infect potato (Solanum tuberosum L.). Arracacha virus A and papaya mosaic virus, ulluco isolate (PapMV-U) infect other crops. PLRV, one of the most damaging potato viruses, was detected in healthy potato plants growing in...