Lichen-forming fungal proteins have been seldom searched due to many difficulties in their extraction. Phenols, quinones, proteases, and other components released during cell disruption have been known to be the greatest challenges related to protein extraction from lichens. To overcome these problems and maintain good electrophoretic resolution and high protein concentration, an extraction buf...

a highly efficient method for the oxidation of aldehydes to carboxylic acids using polyvinylpolypyrrolidone supported - boron trifluoride (pvpp-bf3) in the presence of 35% hydrogen peroxide has been developed in acetonitrile at 60 °c. this procedure cleanly oxidizes variety of aldehydes to the corresponding carboxylic acids. oxidative esterification of benzaldehyde utilizing pvpp-bf3/h2o2(35%) ...

Amino acid conjugates of IAA can be chromatographed on polyvinylpolypyrrolidone (PVPP) using either acidic methanol or aqueous buffers as eluents. In the aqueous system, elution of the compounds is pH-dependent, and the pattern obtained suggests that hydrophobic interactions contribute substantially to the chromatographic behavior of IAA peptides on PVPP. Purification of soybean seed extracts b...

Isolation of RNA from recalcitrant tree tissues has been problematic due to large amounts of secondary metabolites and interfering compounds in their cells. We have developed an efficient RNA extraction method, which yielded high-quality RNA preparations from tissues of the lychee tree. The method reported here utilized EDTA, LSS, and CTAB to successfully inhibit RNase activities. It was found ...

Ethylene-forming enzyme (EFE) was isolated from apple (Malus domestica Borkh. cv Golden Delicious) fruit tissue. The enzyme activity in the homogenate is associated with the pellet fraction and can be solubilized with Triton X-100 or polyvinylpolypyrrolidone. The solubilized enzyme system resembles the in vivo system in that it exhibits a low K(m) (17 micromolar) for its substrate 1-aminocyclop...

−− Clarification of apple juice by flocculation and precipitation with bentonite and gelatin is explained on a more systematic basis, essentially through the determination of turbidity and zetapotential. Apple juice was also treated with Polyvinylpolypyrrolidone (PVPP) to remove total polyphenol. Gelatin-particle complex was evaluated as the increase in juice turbidity after the adding of tanni...

J. Inst. Brew. 111(1), 20–25, 2005 Proanthocyanidins have been characterized and levels compared in an ale and a lager using normal-phase liquid chromatography– mass spectroscopy (LC/MS) after a pre-concentration step employing Sephadex LH-20 chromatography. Twenty proanthocyanidins were identified including both non-galloylated and galloylated forms of the monomers, dimers and trimers. Oligome...