Abstract Background Human noroviruses are one of the main causes foodborne illnesses and represent a serious public health concern. Rapid sensitive assays for human norovirus detection undoubtedly necessary clinical diagnosis, especially in regions without more sophisticated equipment. Method The rapid reverse transcription recombinase-aided amplification (RT-RAA) is fast, robust isothermal nuc...

DNA products generated from a region of the measles virus genome by three RNA reverse transcription and amplification methods were cloned and sequenced, and the results were compared in order to evaluate the methods' relative fidelities. The methods were: (i) reverse transcription followed by a nested polymerase chain reaction (RT-nPCR), (ii) a combined RT-PCR using rTth polymerase and (iii) nu...

The present study was undertaken to analyze the expression pattern of estrogen receptor 1 gene (ESR1) in Barbari bucks (fertile and non-fertile) identified on the basis of seminal quality traits and fertility trials. RNA was extracted from the spleen by Trizol method. The expression pattern of ESR1 gene was analyzed using real time polymerase chain reaction (RT-PCR). The expression pattern of E...

Infectious bursal disease (IBD) is an immunosuppressive, acute and highly contagious illness of growing-poultry stock infected with infectious bursal disease virus (IBDV). It is common in Pakistan, causing potential economic losses throughout the year. The objective of the study is to propose a rapid, sensitive and specific diagnostic tool, and compare it with existing commonly used reverse tra...

Abstract Severe acute respiratory syndrome coronavirus 2 causing disease 2019 pandemic is an enveloped virus, showing genome similarity with bat coronavirus. This virus initially infects the upper tract, subsequent spread to lower tract. Despite availability of antigen and antibody detection methods, reverse transcription-polymerase chain reaction (RT-PCR) diagnostic test choice for this novel ...

results our study showed that the sensitivity and specificity of taqman real time pcr was 96% and 95% respectively. these values were 89% and 90, 38% and 100%, 6% and 100% for pcr, culture and microscopy, respectively. conclusions our study showed that sensitivity of taqman real time pcr was higher that pcr, culture and microscopy but specificity of culture and microscopy was more than pcr and ...

Objective: Reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) is one of the time-saving, accurate, and cost-effective alternative methods to real-time polymerase chain reaction (RT-PCR). This study aimed identify robustness a colorimetric RT-LAMP assay kit that we developed, detecting SARS-COV-2 viral RNA within 30 minutes using primer set special N gene against RT-PCR, gold...

Background: The current focus is largely on whole course medical management of coronavirus disease-19 (COVID-19) with real-time polymerase chain reaction (RT-PCR) and radiological features, while the mild cases are usually missed. Thus, combination multiple diagnostic methods urgent to understand COVID-19 fully monitor progression COVID-19.