low seed yield is a limiting factor for cross breeding and hybrid seed production in cyclamen. this study was performed to investigate pollen germination and its relation to cross-pollination success and fruit set in this plant. in order to achieve a high level of pollen germination, the effect of different concentrations of chemical compounds were examined on in vitro pollen germination of cyc...

Germination of pollen grains is a crucial step in plant reproduction. However, the molecular mechanisms involved remain unclear. We investigated the role of PECTIN METHYLESTERASE48 (PME48), an enzyme implicated in the remodeling of pectins in Arabidopsis (Arabidopsis thaliana) pollen. A combination of functional genomics, gene expression, in vivo and in vitro pollen germination, immunolabeling,...

Pollen tubes are an ideal model for the study of cell growth and morphogenesis because of their extreme elongation without cell division; however, the genetic basis of pollen germination and tube growth remains largely unknown. Using the Illumina/Solexa digital gene expression system, we identified 13,017 genes (representing 28.3% of the unigenes on the reference genes) at three stages, includi...

Polarized cell elongation is triggered by small molecule cues during development of diverse organisms. During plant reproduction, pollen interactions with the stigma result in the polar outgrowth of a pollen tube, which delivers sperm cells to the female gametophyte to effect double fertilization. In many plants, pistils stimulate pollen germination. However, in Arabidopsis, the effect of pisti...

Pollen germination of Vitis vinifera L., of the family Vitaceae was examined in fresh and stored pollen upto 48 weeks at different temperature i.e., refrigerator (+4C), freezer (-20C, -30C) and freeze drier (-60C). Pollen stored at low temperature showed better germination percentage as compared to pollen stored at +4C and fresh. Freeze dried pollen (-60C) showed the highest germination percent...

The aim of this study was to determine the effects of different plant growth regulators, NAA (0 mg/l, 50 mg/l and 100 mg/l) and GA3 (0 mg/l, 50 mg/l and 100 mg/l), with various boron concentrations (0 mg/l, 50 mg/l and 100 mg/l) on pollen germination of ‘Şaba’, ‘Rabie’ and ‘Padre’ almond cultivars in 10% sucrose and 1% agar medium at 24°C in dark conditions. The results showed that different tr...

The focus of this study is to investigate the regulatory role of K(+) influx in Arabidopsis pollen germination and pollen tube growth. Using agar-containing media, in vitro methods for Arabidopsis pollen germination have been successfully established for the first time. The pollen germination percentage was nearly 75% and the average pollen tube length reached 135 microm after a 6 h incubation....

In addition to its importance in studies of plant reproduction and fertility, pollen is as widely employed as a model system of cell growth and development. This work demands robust, reproducible methods to induce pollen germination and morphologically normal growth of pollen tubes in vitro. Despite numerous advantages of Arabidopsis thaliana as a model plant, such experiments on pollen germina...