water crisis and drought are among the most important issues to which the human has been faced particularly in the recent years; hence, investigation of drought is so important in optimal water management. drought has been known as an environmental phenomenon which is inseparable from climate changes which can occur in any geographical region. various definitions have been presented since yet. ...

Background: The main purpose of this study was to evaluate the aboriginal knowledge regarding use medicinally important plant species from Tehsil Malakwal, District Mandi Bahauddin, Pakistan. Methodology: A total 120 inhabitants (75women and 45 men) were interviewed for by using semi structured questionnaire. Data analyzed qualitatively as well quantitative indices Relative Frequency Citation (...

Translesion DNA synthesis (TLS) by specialized DNA polymerases (Pols) is a conserved mechanism for tolerating replication blocking DNA lesions. The actions of TLS Pols are managed in part by ring-shaped sliding clamp proteins. In addition to catalyzing TLS, altered expression of TLS Pols impedes cellular growth. The goal of this study was to define the relationship between the physiological fun...

In addition to RNA polymerases I, II, and III, the essential RNA polymerases present in all eukaryotes, plants have two additional nuclear RNA polymerases, abbreviated as Pol IV and Pol V, that play nonredundant roles in siRNA-directed DNA methylation and gene silencing. We show that Arabidopsis Pol IV and Pol V are composed of subunits that are paralogous or identical to the 12 subunits of Pol...

Mammalian DNA polymerase (pol) is a member of the X-family of DNA polymerases and has striking enzymatic and structural similarities to mammalian DNA pol . Because pol provides two important enzymatic activities for base excision repair (BER), we examined whether pol might also contribute to BER. We used extracts from mouse embryonic fibroblasts representing wild-type and null genotypes for pol...

A new form of DNA polymerase III, termed Pol III star (Pol III(*)), has been purified to homogeneity from Escherichia coli. Pol III(*) is temperature sensitive when isolated from a thermo-sensitive dnaE mutant, as had been described for Pol III. Pol III(*) and Pol III are separable by gel filtration. Pol III(*) utilizes a duplex template containing short gaps with the same catalytic properties ...

Proliferating cell nuclear antigen (PCNA) has been shown to interact with a variety of DNA polymerases (pol) such as pol delta, pol epsilon, pol iota, pol kappa, pol eta, and pol beta. Here we show that PCNA directly interacts with the newly discovered pol lambda cloned from human cells. This interaction stabilizes the binding of pol lambda to the primer template, thus increasing its affinity f...