BACKGROUND The clinical use of autologous platelet concentrates (also known as platelet-rich plasma) on the field of regenerative therapy, in the last decade has been the subject of several studies especially in equine medicine and surgery. The objectives of this study was: 1) to describe and compare the cellular population in whole blood, lower fraction (A) and upper fraction (B) of platelet c...

Repeated exposure to foreign human leukocyte antigens (HLA) present on both platelets and leukocytes often results in refractoriness to transfusions of platelet concentrates. Evidence now exists which suggests that the leukocytes are more immunogenic and that their removal from blood products may delay or prevent the development of refractoriness. In this study, the ability of three blood filte...

There are no large-scale data available describing the increments obtained with platelet concentrates stored for varying durations. Platelet concentrates prepared by standard techniques were stored at 22 degrees C with horizontal agitation in PL-732 bags and administered to clinically stable, nonalloimmunized recipients known to respond well to random donor platelet transfusions. The platelet c...

The main feature of this new approach to platelet freezing is a controlled rate of addition and postthaw removal of 5#{176}/odimethylsulfoxide (DMSO). When DMSO was removed from fresh nonfrozen platelet concentrates before transfusion, no adverse effect on posttransfusion survival was observed. The 51Cr recovery of autologous fresh platelet concentrates in vivo was 65#{176}/o ± 5#{176}/o, while...

Repeated transfusions with platelets from randomly selected donors lead to HLA alloimmunization in about 50% of patients due to lymphocyte contamination of platelet concentrates. Attempts to remove the leukocytes from the platelet concentrates by additional centrifugation steps led to substantial loss of platelets. We report a new procedure for removal of almost all leukocytes with excellent pl...