We report the development of a low-cost method to generate a centimetre-scale periodic array of single plasmid DNA molecules of 11 kilobase pairs. The arrayed DNA molecules are amenable to enzymatic and physical manipulations.

The damage induced in supercoiled plasmid DNA molecules by low energy (<500 eVu) carbon ions has been investigated as a function of ion dose. The production of short linear fragments through multiple double strand breakage is suggested and exponential dose responses for each of the topoisomers are presented.

ABSTRACT Plasmids are common in natural bacterial communities, facilitating evolution via horizontal gene transfer. Bacterial species vary their proficiency to host plasmids: whereas plasmids stably maintained some regardless of selection for plasmid-encoded genes, other species, even beneficial rapidly lost. It is, however, unclear how this variation affects plasmid persistence communities. He...

prepulse inhibition (ppi), the objective measure of sensorimotor gating disturbance has being widely used in animal models of schizophrenia. dopaminergic direct and indirect agonists impair ppi. however, the profile of dopaminergic receptors involved in ppi impairment by dopamine agonists is not clear. by injecting shrna expressing plasmids against dopamine d2 receptor genes (drd2) in the nucle...

  Objective(s): Adeno-associated virus type 2 (AAV2) vectors are widely used for both experimental and clinical gene therapy. A recent research has shown that the performance of these vectors can be greatly improved by substitution of specific surface-exposed tyrosine residues with phenylalanines. In this study, a fast and simple method is presented to generate AAV2 vector helper plasmids encod...

Background: Mycobacterium (M.) bovis is the agent of bovine tuberculosis (TB) in a range of animal species, including humans. Recent advances in immunology and the molecular biology of Mycobacterium have allowed identification of a large number of antigens with the potential for the development of a new TB vaccine. The ESAT-6 and CFP-10 proteins of M. bovis are important structural and function...

In Brazil, the presence of plasmid-mediated AmpC (pAmpC)-producing isolates has been sporadically reported. We evaluated the frequency of pAmpC among 133 Enterobacteriaceae clinical isolates. The bla CMY-2-like gene was detected in a single Klebsiella pneumoniae isolate. In our study, the pAmpC frequency was very low as previously reported.

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