1 Animal Scientist, D.Sc., Adjunct Professor, Department of Animal Science, Universidade Federal de Viçosa, ViçosaMG, 36571-000, CNPq and INCT-Ciência Animal researcher ([email protected]) 2 Animal Scientist, D.Sc., Titular Professor, Department of Animal Science, Universidade Federal de Viçosa, Viçosa-MG, Brazil, 36571-000, CNPq and INCT-Ciência Animal researcher ([email protected]) 3 Agronomy Engi...

This study was carried out to evaluate the plasmid mediated drugs resistance among wound bacteria isolates obtained from (352 swabs specimens) patients attending some hospitals in Kano State (National Orthopaedic Hospital Dala, Aminu Teaching Hospital, Infectious Diseases Bichi General and Wudil Hospital). Swab specimens were aseptically using randomized sampling method cultured on Blood MacCon...

the present study aimed to evaluate the antibiotic resistance pattern of escherichia coli strains isolatedfrom animals to ascertain the levels of antibiotic resistance pervasiveness. a total of 28 e. coli strains wereisolated from faecal samples and the antibiotic resistance pattern of e. coli strains was determined by meansof disc diffusion assay. the resistance pattern determined for all stra...

Quinolone antibiotics have been commonly used to treat cases of multiple antibiotic resistance. Unfortunately, quinolone so much resisted by infectious bacterial agents. This study aimed evaluate the susceptibility some clinical isolates E. coli and determination plasmid-encoded resistance genes.
 Our results showed plasmid quinolone-resistance genes in following prevalence: qnr genes: S (...

pseudomonas aeruginosa is an important life-threatening nosocomial pathogen and plays a prominent role in serious infections in burned patients. the current study was undertaken to characterize p. aeruginosa strains isolated from burned patients in tehran, iran. the study was conducted in a major burn center in tehran, iran in 2007. a total of seventy specimens obtained from different clinical ...

background and purpose: brain-derived neurotrophic factor (bdnf) is present in the hippocampus and cerebral cortex in high concentrations and has an important role in development, maintenance and regeneration of the nervous system. the aim of this study was to produce active bdnf in e.coli using transforming plasmid. methods: to construct an expression plasmid of bdnf, a gene coding for it was ...

shigellosis is an acute gastroenteritis caused by shigella species. the purpose of this study was to determine plasmid profile, antibiotic resistance and phenotypic virulent by congo red between s. flexneri strains. the isolated bacteria were identified by standard bacterial and biochemical methods. plasmids were isolated by alkaline lysis method. antibiotic susceptibility test was performed ac...

Minicircles are non-viral delivery vectors with promising features for biopharmaceutical applications. These plasmid-derived circular DNA molecules that obtained in vivo Escherichia coli by the intramolecular recombination of a parental plasmid, which generates minicircle containing eukaryotic therapeutic cassette interest and miniplasmid prokaryotic backbone. The production process results thu...

Gene regulation circuits control all aspects of the life of plasmids. This review gives an overview of the current orchestration of the circuits that control plasmid replication, plasmid transfer, plasmid segregation and plasmid maintenance.

background: p19 cells are mouse embryonic carcinoma cells which contain pluripotent ability, like stem cells, to differentiate into different cell lines. there are several properties for this cell line that make it a valuable cell model for study of developmental stages. methods: at the first step, pts2-egfp coding sequence which was cloned in pucd2.hygro vector was used for transfection in to ...