New individuals in clonal populations arise through the recruitment of sexual or clonal offspring. The predominance of one type of regeneration over the other has been correlated with different selective environmental pressures. We compared the reproductive mode (sexual through seeds and vegetative through plantlets or detached cladodes) of Opuntia microdasys from three desert habitats of the C...

Embryogenesis was induced from regenerated carrot plantlets in a shorter time and at a higher frequency than that observed previously from hypocotyl explants of carrot seedlings (Masuda et al. J. Plant Physiol. 145, 531 (1995)). Serial observations of embryogenesis from single cells to globular embryos via cell clusters were possible in the conditioned medium. The ability to produce embryos see...

Growth characteristics, oxygen exchange, and carbohydrate and chlorophyll contents were determined 30 days after subculturing of single node-derived plantlets of Solanum tuberosum cv Haig cultivated in vitro. Cultivation conditions were: (a) photomixotrophy in closed vessel, (b) photomixotrophy in closed vessel on medium supplemented with silver thiosulfate, (c) photomixotrophy in aerated vesse...

Background: Lemon verbena (Lippia citriodora H.B.K.) is an aromatic and medicinal plant of family Verbenaceae, which cultivated in the North region of Iran. Objective: This comparative study was carried out on essential oil content and composition of Lippia citriodora H.B.K. from micro-propagated and rooted apical stem cutting plantlets. Methods: The micro-propagated plantlets were obtained ...

Polyploid plantlets, including triploid, tetraploid, and mixoploid, were induced from the European pear (Pyrus communis L.) cultivar ‘Fertility’ by in vitro colchicine treatment of leaf explants. The leaf explants were incubated in 0.4% (w/v) colchicine for 24, 48, or 72 h, then transferred to adventitious shoot-induction medium. Regenerated shoots were pre-selected according to their morpholog...

background: lemon verbena (lippia citriodora h.b.k.) is an aromatic and medicinal plant of family verbenaceae, which cultivated in the north region of iran. objective: this comparative study was carried out on essential oil content and composition of lippia citriodora h.b.k. from micro-propagated and rooted apical stem cutting plantlets. methods: the micro-propagated plantlets were obtained by ...

The aim of our study was to consider the efficiency of randomly amplified polymorphic DNA (RAPD) analysis to show the differences between Rhododendron mutants and control plants. The mutant plantlets were selected due to their better In vitro shoot and root regeneration relative to control in both varieties. Six mutant plantlets from Alfred and seven mutant plantlets from Paars variety were sel...

Morphological, anatomical and physiological features of in vitro plantlets are affected by the environmental conditions experienced. The high water content inside in vitro-formed tissues makes difficult to maintain the original characteristics of samples from in vitro plantlets during the preparation procedures for morphological and anatomical studies. In this paper, micropropagated Vitis vinif...

Plant somatic cells have the remarkable ability to regenerate an entire organism. Many species in the genus Kalanchoë, known as "mother of thousands," develop plantlets on the leaf margins. Using key regulators of organogenesis (STM) and embryogenesis (LEC1 and FUS3) processes, we analyzed asexual reproduction in Kalanchoë leaves. Suppression of STM abolished the ability to make plantlets. Here...

As interest in how to increase biomass production through biotechnological means gains traction, focus is turning towards the use of photoautotrophic micropropagation under elevated levels of carbon dioxide (CO2) to maximize plant growth and productivity. The effect of super-elevated CO2 with cold cathode fluorescent lamps (CCFLs) on the photoautotrophic growth of Oncidesa in vitro has been stu...