The aim of the research work was to study the genetic variability of sunflower (Heliantus annuus L.) seeds collection. From 1500 available genotypes a set of 120 recombinant inbred lines coming from different countries were selected to perform the study. The genetic variability consisted in two types of experiments: in vivo and in vitro cultures. The recombinant inbred lines were classified usi...

The present study was undertaken to investigate the role of banana extract (BE) on synchronous plantlet formation from seeds of Dendrobium lituiflorum Lindl. The seedlings formed on modified Knudson C (KC) medium supplemented with 20% (v/v) BE had significantly high fresh and dry weights. Morphologically uniform plantlets with elongated leaves and well developed roots were formed on KC medium w...

Plant somatic cells have the remarkable ability to regenerate an entire organism. Many species in the genus Kalanchoë, known as "mother of thousands," develop plantlets on the leaf margins. Using key regulators of organogenesis (STM) and embryogenesis (LEC1 and FUS3) processes, we analyzed asexual reproduction in Kalanchoë leaves. Suppression of STM abolished the ability to make plantlets. Here...

abstract to produce embryogenic callus and somatic embryo, whole flower explants were collected at two sampling stages of i, iii. to produce embryogenic callus and somatic embryo, ms medium supplemented with 5 and 10µm 2,4-d and 2µm bap were used. for embryo differentiation, ms medium with 0.5mg/l iba, ms medium without any plant growth regulators, ms medium with 2mg/l iba and 0.2mg/l bap, ms m...

Effect of Potassium Sulfate Application on Some Physiological Characteristics Date Palm Plantlet Zahedi Cultivar (Phoenix dactylifera cv. Zahdei) Under Water Stress

The survival and growth of in vitro plantlets are still problematic for the date palm; thus, application nanotechnology may provide palm that develop faster with enhanced biochemical parameters. Moreover, usage Fe-modified activated carbon (Fe-AC) prepared from pits instead charcoal (AC) tissue culture media during current investigation can be considered an innovative approach. Furthermore, eff...

MicroRNAs (miRNAs) play essential roles in plant development, but the roles in the in vitro plant development are unknown. Leaves of ponkan plantlets derived from mature embryos at in vitro culture conditions were used to sequence small RNA fraction via Solexa sequencing, and the miRNAs expression was analyzed. The results showed that there were 3,065,625 unique sequences in ponkan, of which 0....

to produce embryogenic callus and somatic embryos, tendril explants were collected and cultured in ms medium supplemented with various concentrations of hormones. after one month most of explants produced callus. the highest percent of embryogenic callus obtained in ms medium supplemented with 4.5 µm bap and 1µm 2,4-d. production of somatic embryos was depended on genotype and medium. yaghouti ...

The present study was conducted to investigate the effect of growth regulator NAA on in vitro shoot proliferation, rooting, and plantlet establishment. Among the different concentrations of NAA, the best increase in shoot weight (0.25 g) and shoot number (8.83) were observed from 0.1 mg/I NAA. The highest shoot length (2.60 cm), number of leaves (4.83), number of roots (5.15), and root length (...

Light quality evaluations were conducted on yam (Dioscorea alata L. cultivar 'Oriental' ) plantlets to determine the potential usefulness of manipulation of the light environment in regulating growth. Eight-week old, in vitro grown yam plantlets were transferred to pots and treated in vivo with end-ofday (EOD) red (R) or far-red (FR) light. Also, nodal segments ofD. alata plantlets were culture...