Methods for counterstaining neural tissue that contains fluorescent markers have been developed. Acridine orange is useful for localizing cells that are retrogradely labelled with the fluorescent tracers true blue, bisbenzimide, and nuclear yellow because at low concentrations it yields a green Nissl stain when excited with blue, but not with ultraviolet, light; since the tracers fluoresce only...

Hearing loss, vestibular disorders and damaged eyesight can be the result of axonal degeneration (Int Jour Ped Otrlaryn, 61:1-15; Inst Opthalm, 7:109-125). Improper functioning of the myelin sheath may be responsible for this degeneration, due to its role in conducting chemical and electrical neural signaling. Investigation of myelin degeneration requires understanding of normal myelinogenesis ...

Acid fuchsin and phloxine B are commonly used to stain plant-parasitic nematodes in roots and egg masses on root surfaces, respectively. Both stains can be harmful to both the user and the environment and require costly waste disposal procedures. We developed safer methods to replace both stains using McCormick Schilling red food color. Eggs, juveniles, and adults of Meloidogyne incognita stain...

Materials and Methods Formalin-fixed paraffin-embedded lung SqCC (n=137) and LADC (n=60) tissue microarrays were acquired. DSG3/p40 + Napsin A double stain cocktail (Biocare Medical, Concord, CA), TTF-1 [SPT24] and TTF-1 [8G7G3/1] antibody titers were optimized for IHC. SPT24 was optimally titered at 1:1200. The double stain antibody cocktail was detected by using a polymer double stain detecti...

INTRODUCTION Numerous clinical cases have been reported showing the clinical picture of dementia but not meeting the neuropathological criteria for Alzheimer's dementia (AD). Different methods used to stain senile plaques (SPs) and neurofibrillary tangles (NFTs) might account for this discrepancy. SUBJECTS AND METHODS Here, brains of 11 patients with dementia were examined. Cryosections and p...

A new technique for the staining of the corneal endothelium combines the actions of the vital stain trypan blue and the intercellular stain alizarin red S. The technique is an improvement over former staining methods, because it defines both viable and nonviable cells and thereby permits an investigator to accurately quantify endothelial cell damage.

The color in the pigment of the stain is caused when molecules absorb light in the visible wavelength range (400-780 nm) [10]. The wavelengths that are not absorbed will be reflected instead and are visible to the human eye. For example, red wine stains are red because the molecules absorb every wavelength that is not in the red range, and the red wavelengths (500-600 nm) are then reflected to ...

Macrophages are responsible for removing the extravasated blood that forms a bruise. Aspects of this process, such as how long it takes erythrocytes to be phagocytosed and iron demonstrated using Perls stain, not established. This work sought assess possibility macrophages in cell culture address some unknowns processes resolution Human peripheral monocytes were obtained from buffy coats provid...