It was recently shown that vacuolar ATPase and mitochondrial F1F0-ATPase activities are induced by aluminum (Al) in an Al-resistant cultivar of wheat, suggesting that induction of these enzymes could be an adaptive trait involved in Al resistance. To test this hypothesis, we used the Saccharomyces cerevisiae model system. In yeast, unlike wheat, the activity, transcript and protein levels of mi...

Yme1p, an ATP-dependent protease localized in the mitochondrial inner membrane, is required for the growth of yeast lacking an intact mitochondrial genome. Specific dominant mutations in the genes encoding the alpha- and gamma-subunits of the mitochondrial F(1)F(0)-ATPase suppress the slow-growth phenotype of yeast that simultaneously lack Yme1p and mitochondrial DNA. F(1)F(0)-ATPase activity i...

Abstract The mitochondrial F 1 O ‐ATPase in the presence of natural cofactor Mg 2+ acts as enzyme life by synthesizing ATP, but it can also hydrolyze ATP to pump H + . Interestingly, be replaced Ca , only sustain hydrolysis and not synthesis. When inserts torque generation built chemomechanical coupling between rotating central stalk was reported unable drive transmembrane flux within However, ...

H+-translocating, Mg2+-ATPase was solubilized from vacuolar membranes of Saccharomyces cerevisiae with the zwitterionic detergent N-tetradecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate and purified by glycerol density gradient centrifugation. Partially purified vacuolar membrane H+-ATPase, which had a specific activity of 18 units/mg of protein, was separated almost completely from acid phospha...

We have compared the adenosine triphosphatase (ATPase) activity of mitochondria prepared from wild-type Neurospora crassa and from poky, a maternally inherited mutant known to possess defective mitochondrial ribosomes and reduced amounts of cytochromes aa3 and b. poky contains two distinct forms of mitochondrial ATPase. The first is normal in its Km for ATP, specificity for nucleotides and diva...

Soluble mitochondrial ATPase (F1) isolated from Neurospora crassa is resolved by dodecylsulfate-gel electrophoresis into five polypeptide bands with apparent molecular weights of 59000, 55000, 36000, 15000 and 12000. At least nine further polypeptides remain associated with ATPase after disintegration of mitochondria with Triton X-100 as shown by the analysis of an immunoprecipitate obtained wi...

We have used the tetrameric nature of the fluorescent protein DsRed to cross-link F(1)F(O)-ATPase complexes incorporating a subunit gamma-DsRed fusion protein in vivo. Cells expressing such a fusion protein have impaired growth relative to control cells. Strikingly, fluorescence microscopy of these cells revealed aberrant mitochondrial morphology. Electron microscopy of cell sections revealed t...

The dithiol reagents phenylarsine oxide (PAO) and dibromobimane (DBrB) have opposite effects on the F 1 O -ATPase activity. PAO 20% increases ATP hydrolysis at 50 μM when enzyme activity is activated by natural cofactor Mg 2+ 150 it Ca . PAO-driven activation reverted to basal dithiothreitol (DTE). Conversely, 300 DBrB decreases 25% 50% In both cases, inhibition insensitive DTE. mitochondrial p...

The effect of various alkylguanidines on ion absorption and energy metabolism in oat (Avena sativa cv. Goodfield) roots has been investigated. Of several alkylguanidines tested, octylguanidine was the most effective inhibitor of both K(+) and Cl(-) absorption by excised roots. At 225 mum octylguanidine, the transport of both ions was inhibited within 60 seconds and to a similar extent. Octylgua...

Problem statement: Our previous research has shown that mitochondrial ATPase may have a role in the action of dopaminergic D2 receptors. It is therefore, thought of interest to investigate the effect of bromocriptine, sulpiride or their combination on the mitochondrial and microsomal ouabain sensitive and ouabain insensitive Na/K-ATPase in the liver of rats. Approach: In vivo treatment was carr...