background: degradation of phytic acid to inorganic phosphate in domestic animals’ diets requires thermostable phytase. although basillus subtilis phytase shows a potential to be degraded phytate complex in high temperature, the enzyme activities and yields need to be increased to make them possible for industrial application. methods: the phytase gene from bacillus subtilis dr8886 was isolated...

Traditional supplemental dietary phytase loses activity during steam pelleting. The thermal tolerance and bioefficacy of a phytase product with a thermoprotective coating [coated phytase (C-phytase)] was compared in mash and pelleted diets to a traditional, uncoated phytase (U-phytase) added to a negative control (NC) diet, formulated with reduced dietary Ca and P, and compared with a corn-soyb...

Three experiments were conducted to test the hypotheses that pigs fed diets that are equal in digestible P will perform equally regardless of the concentration of total P in the diets, and that the addition of microbial phytase, distillers dried grains with solubles (DDGS), or a combination of phytase and DDGS will result in a reduction in P excretion. In Exp. 1, a P-free diet and 6 diets conta...

Two studies with growing pigs were conducted using the regression analysis technique to estimate the true digestibility of P (TPD) in canola (CM) and soybean (SBM) meals, and quantify the effect of microbial phytase on TPD of CM and SBM. In each study, 48 (Exp. 1) or 36 (Exp. 2) 17-kg barrows were assigned to 6 dietary treatments arranged in a 3 x 2 factorial of 3 graded levels of CM (Exp. 1) o...

Both 1,25-dihidroxyvitamin D3 and phytase are known to improve phosphorus utilization in broilers(Biehl and Baker,J Nutr 1997). Solanum glaucophyllum(Sg) is a plant containing high amounts of 1,25dihydroxyvitamin D bound to a glycoside. It may be an economical source of 1,25-dihydroxyvitamin D for animal feeds. We examined the effect of Sg, with and without phytase, on phosphorus utilization in...

This experiment was conducted to investigate the effects of supplemental phytase in a corn-wheatsoybean meal basal diet on phosphorus (P) digestibility and performance of broiler chicks. 378 one-day old broiler chicks (Ross 308) were allocated to 3×3 factorial arrangements with three levels of phytase enzyme (0, 500 and 1000 FTU/kg) and three levels of non-phytate P (100, 80 and 60% of NRC requ...

A study was conducted to study the effects of thermostable phytase supplementation on the growth performance and Ca and P metabolism of broilers for 21 d. 1-day-old Arbor Acres chicks with similar initial body weight were randomly divided into 3 treatments consisting 8 pens of 10 chicks per pen. The control group was given maize-soybean meal basal diet, and the other two groups were fed the bas...

The regression method was used in a 14-d broiler chicken study to determine the true ileal digestibility of amino acid (AA) and protein utilization in canola meal (CM, 388 g of CP/kg) without or with added phytase. Experimental treatments consisted of 2 factors, phytase at 2 levels (0 or 1,500 phytase units/kg) and CM at 3 levels (125, 250, or 375 g/kg). Birds received a standard starter diet f...

A study was conducted to evaluate the impact of feeding soybean meal (SBM) from low-phytate (LP) or traditional phytate (TP) soybeans on performance and excretions from growing swine. Ninety-six crossbred barrows (initial BW = 18 +/- 0.3 kg) were allocated by BW to 24 pens and fed 1 of 4 treatment diets: TP SBM without supplemental phytase; TP SBM plus 500 phytase units of phytase/kg, as-fed ba...

Bacillus amyloliquefaciens DS11 phytase (DS11 phytase) and Aspergillus ficuum phytase (AF phytase) activities were investigated by measuring the release of phosphate from phytate in animal feedstuff such as wheat bran, corn meal, soybean meal and rice flour at pH 5 and 7. In all the tested feedstuff, the enzymatic activity of DS11 phytase was more active at pH 7, but that of AF phytase was more...