Organic dyes commonly used for planar laser-induced fluorescence (PLIF) are susceptible to photobleaching under laser excitation. Previous work has shown that photobleaching can induce significant errors in laser-induced fluorescence measurements made with single-point techniques. This paper presents an analytical model and experimental results that quantify the extent of photobleaching in the ...

Dissolved organic matter (DOM) is a major light-absorbing substance, responsible for much of the color in water bodies. When sunlight energy is absorbed by DOM, some color can be lost by the process of photobleaching. We measured rates of DOM photobleaching in thirty lakes that varied greatly in color, trophic status and ionic composition. Loss of color (measured as absorbance at 440 nm and exp...

Eukaryotic cells are composed of an intricate system of internal membranes that are organized into different compartments--including the endoplasmic reticulum (ER), the nuclear envelope, the Golgi complex (GC), lysosomes, endosomes, caveolae, mitochondria, and peroxisomes--that perform specialized tasks within the cell. The localization and dynamics of intracellular compartments are now being s...

We report the acquisition and analysis of spectrally resolved photobleaching data from a model system designed to exhibit FRET. Spectrally resolved photobleaching can be used to determine the presence of FRET in these systems and to investigate multi-step mechanisms of energy transfer. The model system was a previously described set of fluorescent beads consisting of a system of six fluorophore...

This study investigates the effect of photobleaching on laser-induced fluorescence (LIF) measurements. Photobleaching causes the fluorescence to be velocity-dependent, which is undesirable if quantitative measurements are being made. To quantify this effect, simultaneous and coincident measurements of fluorescence and velocity were made within the measuring volume of a three-beam laser-Doppler ...

We studied the photobleaching of a library of boron dipyrromethene (BODIPY) derivatives with a range of electron densities, and found that the photobleaching rate is influenced by the electron-withdrawing capacity of the substituents. Electron-deficient BODIPYs generated less singlet oxygen, were less reactive to singlet oxygen, and were highly resistant to photobleaching. We confirmed the util...