We report the first observation of metal-enhanced phosphorescence (MEP) at low temperature. Silver island films (SiFs) in close proximity to Rose Bengal, significantly enhance the phosphorescence emission intensity. The enhanced phosphorescence intensity is 5-fold brighter from SiFs as compared to a glass control sample at 77 K. In addition, enhanced fluorescence emission and spectral narrowing...

The reversible denaturation of Escherichia coli alkaline phosphatase (AP) was followed by monitoring changes in enzymatic activity as well as by measurements of the time-resolved room temperature phosphorescence from Trp 109. It is well known that the denaturants, ethylene diamine tetraacetic acid (EDTA), acid and guanidine hydrochloride (GdnHCl) inactive AP by different mechanisms as reflected...

Phosphorescence has been detected from the dimeric primary electron donor in Rhodobacter sphaeroides and Rhodopseudomonas viridis reaction centers. For Rb. sphaeroides the emission maximum occurs at 1318 + 2 nm at 20 K and is nearly independent of temperature. The emission linewidth (full width at half maximum) is 240 + 20 cm-! at 20 K and increases to 580 + 40 cm-i at 280 K. The phosphorescenc...

We describe the red phosphorescence exhibited by a class of structurally simple benzo[2,1,3]thiadiazoles at room temperature. The photophysical properties of these molecules in deoxygenated cyclohexane, including their absorption spectra, steady-state photoluminescence and excitation spectra, and phosphorescence lifetimes, are presented. Time-dependent density functional theory calculations wer...

Two new AIE-active dinuclear Schiff base Ir(III) complexes exhibit highly reversible piezochromic phosphorescence behaviour enabling the construction of a re-writable phosphorescence data recording device.

An experimental investigation of the phosphorescence properties of liquid and vapour acetone is presented with the goal of introducing a novel technique for improved two-phase flow visualization. Commonly applied laser induced fluorescence (LIF) investigations of two-phase flows are challenging, in particular because of the large disparity in fluorescence intensity between the two phases and th...

The room temperature phosphorescence of native human carbonic anhydrase (CA), and several mutants of this enzyme has been investigated. In these mutants the seven tryptophan residues in the native protein have sequentially been replaced by cysteine or phenylalanine. All of the mutants as well as native CA show room-temperature tryptophan phosphorescence (RTP) spectra. Surprisingly, only small d...

Room temperature phosphorescence emission of erythrosin B in sol±gel silica is studied over the oxygen pressure range from 10ÿ6 to 1000 mbar. Quenching by oxygen is signi®cant over the entire range. The Stern±Volmer (SV) intensity data can be very well ®tted using the two-site model. The temperature dependence of the phosphorescence and the delayed ̄uorescence are examined from ÿ173 to 2008C at...

A system suitable for real-time measurement of oxygen partial pressure (p02) in respiratory gas (oxigraphy) that is based on oxygen's ability to quench the phosphorescence of a lumiphore is described A description of currently used p02 technologies is provided followed by a description of the luminescence quenching p02 technology. A comparison of the technologies is made. The description of lum...

Partial mercuration of DNA, such that roughly one mercuric ion is bound for every two thymine residues, results in an enhancement of the phosphorescence by a factor of ten and a small enhancement of the photosteady thymine dimer yield. Complete mercuration of DNA [one Hg(II) added per momoner unit] results in quenching of the phosphorescence intensity and an inhibition of thymine dimer producti...