The subcellular and submitochondrial localization of CTP:phosphatidate cytidylyltransferase is altered in the Morris 7777 hepatoma. Mitochondria in this poorly differentiated tumor are the principal sites of CDP-diacylglycerol synthesis, in contrast to normal rat liver where the endoplasmic reticulum is most active. This enzyme activity was increased 1 7-fold in the outer mitochondrial membrane...

Apparatus and technique for the microelectrophoresis of heparin on an agarose gel are described in detail. Four brands of commercial gut mucosa heparin were tested. All had a fast component and a slow component, the latter migrating 0.88 ( 0.86 to 0.91) relative to the fast band. The difference in the rate of migration of the commercial preparations ( 1.02 to 0.97 relative to the arbitrary stan...