نتایج جستجو برای: phosphate gradient method
تعداد نتایج: 1815145 فیلتر نتایج به سال:
the effect of boundary conditions on the solution of the inverse problem of identifying the geometry and location of a cavity inside an elastic solid body using displacement measurements obtained from a tension test is investigated. the boundary elements method (bem) coupled with the genetic algorithm (ga) and the conjugate gradient method (cgm) are implemented in this identification problem. a...
This new method for fractionation of serum alkaline phosphatase isoenzymes is based on isoelectric focusing on a mixed-type polyacrylamide support containing an immobilized pH gradient with a superimposed carrier-ampholyte gradient. All known forms of alkaline phosphatase are separated in an Immobiline pH 3.5-6.0 gradient, the sample being applied into pockets cast on a pH 8.0 plateau. Sharp zy...
A method for preparing highly purified glutaminase (EC 3.5.1.2, L-glutamine amidohydrolase) from pig renal cortex is described. The main steps consist of sodium sulfate fractionation followed by alternative solubilization by dialysis against Tris-HCI buffer and precipitation with phosphateborate. The linal enzyme preparation contains no impurities which could be detected by sucrose gradient cen...
A simple and rapid method is described for separation of T-even bacteriophage deoxyribonucleic acid (DNA) from host (Escherichia coli) DNA by hydroxyapatite column chromatography with a shallow gradient of phosphate buffer at neutral pH. By this method, bacteriophage T2, T4, and T6 DNA (but not T5, T7, or lambda DNA) could be separated from host E. coli DNA. It was found that glucosylation of t...
Trends in groundwater pollution with nitrate and phosphate may be an indication of water resources management. The aims of this research were to determine changes in nitrate and phosphate concentration and changes in spatial variability patterns of nitrate and phosphate and distribution over a 5-year period. To do this, 100 agricultural wells were sampled in the years 2006, 2010 and 2011, and a...
Streptococcus bovis JB1 cells were able to transport serine, threonine, or alanine, but only when they were incubated in sodium buffers. If glucose-energized cells were washed in potassium phosphate and suspended in potassium phosphate buffer, there was no detectable uptake. Cells deenergized with 2-deoxyglucose and incubated in sodium phosphate buffer were still able to transport serine, and t...
مسأله بهینه سازی با قیود محدب بعنوان یکی از مسائل مهم و اساسی در بهینه سازی مطرح میباشد. مسائل برنامه ریزی خـطی حالــت خاصی از چنین مسائلی می باشند. در اینجا مسأله بهینه سازی غیرخــطی با قیود محدب، در حالتی بیان میشود که قیود محدب را بصورت قیود کراندار ساده داشته باشیم. یعنی، تنها متغیرهای مسأله از بالا و پایین کراندار باشند. در چند دهه اخیر روش های بسیاری (روش های یکنوا و غیریکنوا ) برای حل ای...
in this work, a new, simple and fast method for the solid phase extraction-spectrophotometric determination of phosphate using cetyltrimethyl ammonium bromide immobilized on alumina-coated magnetite nanoparticles (ctab@acmnps) has been developed. the determination of phosphate is based on the molybdenum blue method which was monitored at λmax of 810 nm. mnps and acmnps characterized by sem, vsm...
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