Candida utilis has been shown to contain 4.7 pmol of cobalamin per g of wet cell paste. Purification of the cobalamin showed it to be a mixture of methylcobalamin and adenosylcobalamin. Two cobalamin-dependent enzyme systems have been found in the yeast: methylcobalamin-dependent methionine biosynthesis and leucine 2,3-aminomutase. The cobalamin extracted from the yeast is as effective as authe...

D-Lysine 5,6-aminomutase from Clostridium sticklandii catalyzes the 1,2-shift of the epsilon-amino group of D-lysine and reverse migration of C5(H). The two genes encoding 5,6-aminomutase have been cloned, sequenced, and expressed in Escherchia coli. They are adjacent on the Clostridial chromosome and encode polypeptides of 57. 3 and 29.2 kilodaltons. The predicted amino acid sequence includes ...

S-Adenosylmethionine (SAM) and an iron-sulphur centre function in place of adenosylcobalamin. Because of its coenzyme BJike role in this reaction, SAM was originally described by H. A. Barker as ‘A poor man’s adenosylcobalamin’ [4]. The conversion of lysine into P-lysine by lysine 2,3-aminomutase proceeds without exchange of solvent protons with substrate hydrogen, and by the stereochemistry il...

Lignin is an underutilized sustainable source of aromatic compounds. To valorize the low-value lignin monomers, we proposed efficient strategy, involving enzymatic conversion from trans-p-hydroxycinnamic acids to generate valued-added canonical and non-canonical amino acids. Among them, β-amino are recognized as building blocks for bioactive natural products pharmaceutical ingredients due their...

In most bacteria, in archaea and in plants, the general precursor of all tetrapyrroles, 5-aminolaevulinic acid, is formed by two enzymes. The initial substrate, glutamyl-tRNA, is reduced by NADPH-dependent glutamyl-tRNA reductase to form glutamate 1-semialdehyde. The aldehyde is subsequently transaminated by glutamate-1-semialdehyde 2,1-aminomutase to yield 5-aminolaevulinic acid. The enzymic m...

C-1027 is an enediyne antitumor antibiotic composed of a chromophore with four distinct chemical moieties, including an (S)-3-chloro-4,5-dihydroxy-beta-phenylalanine moiety that is derived from l-alpha-tyrosine. SgcC4, a novel aminomutase requiring no added co-factor that catalyzes the formation of the first intermediate (S)-beta-tyrosine and subsequently SgcC1 homologous to adenylation domains...

Background: Taxol is one of the most important anti-cancer drugs, which is obtained from yew trees (<span style="font-variant: normal; ...

D-Ornithine aminomutase from Clostridium sticklandii catalyzes the reversible rearrangement of d-ornithine to (2R,4S)-2,4-diaminopentanoic acid. The two genes encoding d-ornithine aminomutase have been cloned, sequenced, and expressed in Escherichia coli. The oraS gene, which encodes a protein of 121 amino acid residues with M(r) 12,800, is situated upstream of the oraE gene, which encodes a pr...

7 ABSTRACT: β-Amino acids are emerging as an important class of 8 compounds in medicinal chemistry. β-Aryl-β-alanines show antiepilepto9 genesis activity, while others have been used to synthesize antibiotic β10 peptides. To assess the utility of a methylidene imidazolone-dependent 11 Pantoea agglomerans phenylalanine aminomutase (PaPAM) for making 12 non-natural β-amino acids, we surveyed the ...