The role of H(2)O(2) and protein thiol oxidation in oxidative stress-induced epithelial paracellular permeability was investigated in Caco-2 cell monolayers. Treatment with a H(2)O(2) generating system (xanthine oxidase + xanthine) or H(2)O(2) (20 microM) increased the paracellular permeability. Xanthine oxidase-induced permeability was potentiated by superoxide dismutase and prevented by catal...

<p>The present work aimed to carry out the phytochemical analysis and antioxidant activity of hydro-methanolic, ethyl acetate butanolic extracts Dianthus sylvestris subsp, aristidis (Batt,) Greuter & Burdet leaves. Phenolics, flavonoids flavonols contents were measured by Folin-Ciocalteu reagent aluminum chloride (AlCl3) method respectively. Phytochemical was determined using LC-E...

HE IMPORTANCE OF THE QUANTITATIVE ANALYSIS OF SERUM IRON has been long established (1, 2, 3), while the worthiness of the determination of serum copper is becoming of increasing interest (4, 5, 6). Therefore, it would be of real value if the concentration of both metabolically interrelated constituents of serum (7, 8, 9) could be determined accurately. It has recently been shown that copper and...

1. Beef kidney 3-hydroxyanthranilate oxygenase was purified in the inactive form, free of bound ferrous ion. Its properties are very similar to those of the liver enzyme. 2. The enzyme in the early stages of purification could be activated by acidification to about pH 4 in the presence of ferrous salts. For the most purified preparations, a sulfhydryl compound, as well as acid and iron, was req...

-N=C&N-, have been known to react as bidentate ligands with certain metal ions such as ferrous, cuprous, and cobaltous, to give colored complex species (1-6). This effect was first noticed with the ferrous ion ( I ) , and since this complex of a given compound is generally of a more intense color than are those with other ions (7), the atomic configuration in question was given the trivial name...

High levels of iron-nitrogen doped porous carbon materials are obtained from MOF-253 using a step-by-step post-synthetic modification strategy. MOF-253 possessing open 2,2'-bipyridine nitrogen sites not only serves as a precursor but also provides chelate bonding sites for Fe2+. Followed by further impregnation of 1,10-phenanthroline, high surface area porous carbon materials are obtained. For ...

Fe(III) and Fe(II) ions were separated and determined via in-column and post-column reactions with salicylic acid and 1,10-phenanthroline by microcolumn ion chromatography with UV-Vis detection. The separation could be achieved on a silica microcolumn using an aqueous ethanol solution of salicylic acid as eluent. The effluent from the column was then mixed with aqueous ethanol solution of 1,10-...