Background: The utilization of methane for production of Poly-β-hydroxybutyrate (PHB) not only cuts the emissions of greenhouse gases but also greatly reduces PHB production cost. Objectives: The aim of this study was to determine the effects of gas-phase conditions on PHB production by Methylosinus trichosporium OB3b. Ma...

polyhydroxyalkanoates (phas) are bioplastics derived from renewable resources such as vegetable oils, corn starch, or microbes. the polyhydroxybutyrate (phb) is a short-chain-length pha, and the most important bioplastic produced by certain microorganisms in the presence of excess carbon sources. in this study batch cultivation of alcaligenes eutrophus with the aim of increasing phb production ...

Expression of prohibitin 1 (PHB), a multi-functional protein in the cell, is decreased during inflammatory bowel disease (IBD). Little is known regarding the regulation and role of PHB during intestinal inflammation. We examined the effect of tumor necrosis factor alpha (TNF), a cytokine that plays a central role in the pathogenesis of IBD, on PHB expression and the effect of sustained PHB exp...

Poly(3-hydroxybutyrate) (PHB) production and mobilization in Ralstonia eutropha are well studied, but in only a few instances has PHB production been explored in relation to other cellular processes. We examined the global gene expression of wild-type R. eutropha throughout the PHB cycle: growth on fructose, PHB production using fructose following ammonium depletion, and PHB utilization in the ...

Polyhydroxyalkanoate (PHA) is a biodegradable material with many potential biomedical applications, including medical implants and drug delivery. This study developed a system for screening production strains in order to optimize PHA production in Cupriavidus taiwanensis 184, 185, 186, 187, 204, 208, 209 and Pseudomona oleovorans ATCC 29347. In this study, Sudan black B staining, Infrared (IR) ...

BACKGROUND Polyhydroxyalkanoates (PHAs) are a group of biodegradable plastics that are produced by a wide variety of microorganisms, mainly as a storage intermediate for energy and carbon. Polyhydroxybutyrate (PHB) is a short-chain-length PHA with interesting chemical and physical properties. Large scale production of PHB is not wide-spread mainly due to the downstream processing of bacterial c...

PHB is a reported oncogene and tumor suppressor in gastric cancer. Here, we evaluated whether the PHB copy number and the rs6917 polymorphism affect its expression in gastric cancer. Down-regulation and up-regulation of PHB were observed in the evaluated tumors. Reduced expression was associated with tumor dedifferentiation and cancer initiation. The T allele of the rs6917 polymorphism was asso...

We propose a new Per Hop Behavior (PHB) for differentiated services in IP networks. In this PHB, each network subscriber (or flow) receives a bandwidth allocation proportional to the subscribed information rate. This new PHB is called "Proportional Forwarding PHB" or PF PHB. The marking mechanism at the source or ingress router and the queuing and discard behavior at core routers are clearly de...

Recombinant Escherichia coli XL1-Blue harboring a high-copy-number plasmid containing the Alcaligenes eutrophus polyhydroxyalkanoate synthesis genes could efficiently synthesize poly(3-hydroxybutyrate) (PHB) in a complex medium containing yeast extract and tryptone but not in a defined medium. One of the reasons for the reduced PHB production in a defined medium was thought to be severe filamen...

Differences in carbon assimilation pathways and reducing power requirements among organisms are likely to affect the role of the storage polymer poly-3-hydroxybutyrate (PHB). Previous researchers have demonstrated that PHB functions as a sole growth substrate in aerobic cultures enriched on acetate during periods of carbon deficiency, but it is uncertain how C(1) metabolism affects the role of ...