The use of luminescence techniques for detection in HPLC has high potential. These techniques are especially useful when extremely high sensitivity and/or selectivity are required, and they can be nicely adapted to the demands of miniaturised HPLC. Three aspects are discussed in the present paper: (i) on-line post-column derivatisation in HPLC with conventional fluorescence detection; (ii) the ...

In this work, using benzo(a)pyrene (BaP) as marker, the analytical merits of on-line flow-injection cloud-point extraction (FI CPE), including preconcentration factor, extraction efficiency, sample throughput, and analysis time were evaluated by use of peroxyoxalate chemiluminescence (CL) detection. Moreover, by detailed discussion of several preconcentration conditions for traditional and on-l...

Peroxyoxalate system includes an oxalate ester, peroxide and a fluorescer, The energy resulted from the reaction of oxalate ester and peroxide is transferred to fluorescer and cause it to fluorsc. The effect of reactants chemical structure, their concentration, solvent media (polarity and viscosity) and the temperature, on the intensity of the illuminated light with respect to time have bee...

A simple and sensitive procedure for the determination of gentamicin is presented, based on the use of the peroxyoxalate chemiluminescent (PO-CL) system in presence of imidazole as a catalyst. The gentamicin has to be previously derivatized with o-phthaladehyde (OPA) in order to obtain a fluorophore, which participates in the PO reaction, producing a CL emission proportional to the gentamicin c...