we searched for novel chinese hamster ovary (cho) cell mutants defective in peroxisome biogenesis by an improved method using peroxisome targeting sequence (pts) of pex3p (amino acid residues 1–40)-fused enhanced green fluorescent protein (egfp). from mutagenized tkaeg3(1–40) cells, the wild-type cho-k1 stably expressing rat pex2p and of rat pex3p(1–40)-egfp, numerous cell colonies resistant to...

UNLABELLED The cucumber anthracnose fungus Colletotrichum orbiculare forms specialized cells called appressoria for host penetration. We identified a gene, FAM1, encoding a novel peroxin protein that is essential for peroxisome biogenesis and that associates with Woronin bodies (WBs), dense-core vesicles found only in filamentous ascomycete fungi which function to maintain cellular integrity. T...

Homologs of peroxin 16 genes (PEX16) have been identified only in Yarrowia lipolytica, humans (Homo sapiens), and Arabidopsis (Arabidopsis thaliana). The Arabidopsis gene (AtPEX16), previously reported as the SSE1 gene, codes for a predicted 42-kD membrane peroxin protein (AtPex16p). Lin et al. (Y. Lin, J.E. Cluette-Brown, H.M. Goodman [2004] Plant Physiol 135: 814-827) reported that SSE1/AtPEX...

A family of proteins collectively referred to as ‘‘peroxins’’ are involved variously in the biogenesis of peroxisomes. Recent studies on the characterization of a T-DNA mutant of a putative plant peroxin gene in Arabidopsis, AtPEX10, led to conclusions that the peroxin gene product, AtPex10p, is required for normal embryo development and viability, possibly through its involvement in the format...

Peroxins are genetically defined as proteins necessary for peroxisome biogenesis. By screening for reduced response to indole-3-butyric acid, which is metabolized to active auxin in peroxisomes, we isolated an Arabidopsis thaliana peroxin4 (pex4) mutant. This mutant displays sucrose-dependent seedling development and reduced lateral root production, characteristics of plant peroxisome malfuncti...

Peroxins are genetically defined as proteins necessary for peroxisome biogenesis. By screening for reduced response to indole-3-butyric acid, which is metabolized to active auxin in peroxisomes, we isolated an Arabidopsis thaliana peroxin4 (pex4) mutant. This mutant displays sucrose-dependent seedling development and reduced lateral root production, characteristics of plant peroxisomemalfunctio...

A variety of metabolic pathways are sequestered in peroxisomes, conserved organelles that are essential for human and plant survival. Peroxin (PEX) proteins generate and maintain peroxisomes. The PEX1 ATPase facilitates recycling of the peroxisome matrix protein receptor PEX5 and is the most commonly affected peroxin in human peroxisome biogenesis disorders. Here, we describe the isolation and ...