Methylhydrazine oxidation promoted by horseradish peroxidase-H2O2 or ferricyanide led to the generation of high yields of methyl radicals and to the formation of 7-methylguanine and 8-methylguanine upon interaction with calf thymus DNA. Methyl radicals were identified by spin-trapping experiments with alpha-(4-pyridyl-1-oxide)-N-tert-butyl nitrone and tert-nitrosobutane. The methylated guanine ...

To develop a new more accurate spectrophotometric method for detecting monoamine oxidase inhibitors from plant extracts, a series of amine substrates were selected and their ability to be oxidized by monoamine oxidase was evaluated by the HPLC method and a new substrate was used to develop a peroxidase-linked spectrophotometric assay. 4-(Trifluoromethyl) benzylamine (11) was proved to be an exc...

To develop a new more accurate spectrophotometric method for detecting monoamine oxidase inhibitors from plant extracts, a series of amine substrates were selected and their ability to be oxidized by monoamine oxidase was evaluated by the HPLC method and a new substrate was used to develop a peroxidase-linked spectrophotometric assay. 4-(Trifluoromethyl) benzylamine (11) was proved to be an exc...

To develop a new more accurate spectrophotometric method for detecting monoamine oxidase inhibitors from plant extracts, a series of amine substrates were selected and their ability to be oxidized by monoamine oxidase was evaluated by the HPLC method and a new substrate was used to develop a peroxidase-linked spectrophotometric assay. 4-(Trifluoromethyl) benzylamine (11) was proved to be an exc...

BACKGROUND Enzyme based remediation of wastewater is emerging as a novel, efficient and environmentally-friendlier approach. However, studies showing detailed mechanisms of enzyme mediated degradation of organic pollutants are not widely published. RESULTS The present report describes a detailed study on the use of Soybean Peroxidase to efficiently degrade Trypan Blue, a diazo dye. In additio...

Aiming at studying cooperativity effects between amino acids in easily accessible protein models, we have explored the chemistry of peptide dendrimers, which obtain as pure products by solid-phase synthesis using a branching diamino acid such lysine every second or third position sequence, followed reverse-phase HPLC purification. This article reviews discoveries driven combinatorial library an...

Background: In this research, we investigated the antioxidant phytochemicals, antimicrobial and antibiofilm properties of methanolic leaf extract from Rhazya stricta.Methods: The main components R. stricta were confirmed through HPLC techniques. activity was examined by DPPD, ABTS, SOD Glutathione peroxidase assays.Results: results showed that better free radical scavenging with IC50 value 74.2...

Sensitive monitoring of the target products during biosynthesis process is crucial, and facile analytical approaches are urgently needed. Herein, phosphatidylserine (PS) was chosen as model target, a colorimetric aptasensor developed for rapid quantitation in samples. A chimeric aptamer constructed with two homogeneous original PS aptamers. Specific recognition between results desorption from s...

The Arctic mutation interferes with processing of the amyloid precursor protein. The Arctic Alzheimer mutation favors intracellular Aȕ production by making APP less available to Į-secretase. Journal of Neurochemistry, in press.ȕ oligomers are inefficiently measured by enzyme-linked immunosorbent assay. and Johansson AS. Docosahexaenoic acid stimulates non-amyloidogenic APP processing resulting ...

BACKGROUND Previous methods to measure glycohemoglobin (GHb) have been time-consuming or imprecise; we therefore developed a new enzymatic assay for GHb. METHODS Blood cells were first hemolyzed, and hemoglobin was digested with protease to yield fructosyl amino acid. Fructosyl amino acid oxidase acts on the fructosyl amino acid and generates hydrogen peroxide, which reacts with chromogens in...