Background: ROS production is a well-established phenomenon during sperm cryopreservation. Therefore, supplementation of freezing media with antioxidant is an inevitable event. The aim of this study was to assess whether a cell permeable superoxide dismutase agent such as MnTE, can further improve the quality of frozen/ thawed semen sample using a commercially optimized sperm cryopreservation m...

Arginine-rich membrane-permeable peptides (APPs) can be delivered to cells by forming complexes with various membrane-impermeable bioactive molecules such as proteins. We recently reported on the preparation of guanidinylated chitosan (GCS) that mimics arginine peptides, using chitosan, a naturally occurring cationic polysaccharide, and confirmed it enhances protein permeability in an vitro cel...

Within an electrodialysis unit, the solutions are separated by alternately arranged anion exchange membranes (permeable only for anions) and cation exchange membranes (permeable only for cations). The membranes are arranged parallel to one another to form an electrodialysis stack. When a potential is applied across the selectively permeable membrane stack, anions selectively move through the an...

Voltage-gated sodium selective ion channel Na(V)1.5 is expressed in the heart and the gastrointestinal tract, which are mechanically active organs. Na(V)1.5 is mechanosensitive at stimuli that gate other mechanosensitive ion channels. Local anesthetic and antiarrhythmic drugs act upon Na(V)1.5 to modulate activity by multiple mechanisms. This study examined whether Na(V)1.5 mechanosensitivity i...

Numerous biological assays and pharmacological studies on various higher plant tissues have led to the suggestion that voltage-dependent plasma membrane Ca2+ channels play prominent roles in initiating signal transduction processes during plant growth and development. However, to date no direct evidence has been obtained for the existence of such depolarization-activated Ca2+ channels in the pl...

The method is cheap and quick. Due to lowering the temperature of solution's glass transition the permeable cryoprotectants prevents the actual freezing of solution and allows to maintain its some flexibility in a glassy phase. Vitrification without permeable cryoprotectants allows to avoid the cryoprotectants toxicity and osmotic stress; the damage of plasmatic and mitochondrial membrane durin...