conclusions periodic evaluation of antimicrobial susceptibility for c. perfringens should be performed. harboring of enterotoxigenic c. perfringens in individuals not necessarily results in diarrhea. results of 136 stool samples including diarrhea [48] and non-diarrhea [88] ones, 83 (61.02%) c. perfringens were cultured. of these 83, 79 c. perfringens isolates showed the alpha-toxin (phospholip...

Clostridium perfringens is an important cause of enteric diseases in both human and animals. The bacteria produce several toxins which play key roles in the pathogenesis of diseases and are classified into five toxin types, on the basis of the differential production of Alpha, Beta, Epsilon and Iota toxins. In this study a single PCR assay was developed and used for detection of cpb2 gene to id...

The antimicrobial effect of tannic acid and raw material of Acacia nilotica fruits and leaves (tannin-rich plant) on Clostridium perfringens were comparatively evaluated. Different concentrations) 10 mg to 10 μg/ml)of tannic acid; fruits, and leaves were tested on Cl. perfringens. The obtained results revealed that the minimum inhibitory concentrations were 156, 75 and 100 μg/ml for tannic acid...

Clostridium perfringens is a pathogen of great concern in veterinary medicine, because it causes enteric diseases and different types of toxaemias in domesticated animals. It is important that bacteria in tissue samples, which have been collected in the field, survive and for the classification of C. perfringens into the correct toxin group, it is crucial that plasmid-borne genes are not lost d...

AIMS Clostridium perfringens type A causes both clinical and subclinical forms of necrotic enteritis in domestic avian species. In this study the inhibitory effect of hen egg white lysozyme on the vegetative form of Cl. perfringens type A and the production of alpha-toxin in vitro was investigated. METHODS AND RESULTS A micro-broth dilution assay was used to evaluate the minimal inhibitory co...

The enteric microbiota contributes to the pathogenesis of inflammatory bowel disease, but the pathways involved and bacterial participants may vary in different hosts. We previously reported that some components of the human commensal microbiota, particularly Clostridium perfringens (C. perfringens), have the proteolytic capacity for host matrix degradation and reduce transepithelial resistance...

The incidence and numbers of enterotoxigenic Clostridium perfringens in the intestinal contents of cattle, swine and broiler chickens were determined and compared with those of total (enterotoxigenic and nonenterotoxigenic) C. perfringens. The method used for the enumeration of enterotoxigenic C. perfringens consisted of a combination of the most probable number (MPN) method and a nested polyme...

Clostridium perfringens causes various digestive system disease symptoms in pigs. In the present study, 11 C. perfringens isolates were obtained from diarrheic piglets and 18 from healthy piglets. All of the C. perfringens isolates were shown to be type A using a multiplex PCR assay. The β2 toxin gene was detected in 27/29 C. perfringens isolates, i.e., 81% (9/11) of diarrheic piglets and 100% ...

clostridium perfringens is a gram-positive, obligate anaerobic bacterium, which is widely distributed in the environment. c. perfringens is subdivided to 5 groups (types a to e), based on its four major toxin (alpha, beta, epsilon and iota). c. perfringens type b beta toxin causes inflammation and bloody necrotic enteritis. type b related enterotoxaemia is a major problem of veterinary sciences...

An improved selective medium, Tryptose-sulfite-cycloserine (TSC) agar, for the enumeration of Clostridium perfringens is described. It consists of the same basal medium as Shahidi-Ferguson-perfringens (SFP) agar, but with 400 mug of D-cycloserine per ml substituted for the kanamycin and polymyxin. Tolerance of C. perfringens for D-cycloserine, its production of lecithinase, and its ability to r...