Biological systems are often exposed to mechanical perturbations, which may modulate many biochemical processes. Ligand binding involves a wide range of structural changes in the receptor protein, from hinge movement entire domains minor sidechain rearrangements pocket residues. Hydrophobic ligand protein alters system’s vibrational free energy, allowing different conformational states alloster...

Ligand-immobilization to stents and vascular grafts is expected promote endothelialization by capturing flowing endothelial progenitor cells (EPCs). However, the optimized ligand density linker structure have not been fully elucidated. Here, we report that EPCs were selectively captured REDV peptide conjugated with a short linker. The microchannel surface was modified via Gly-Gly-Gly (G3), (Gly...

Abstract Roots provide the plant with water and nutrients anchor it in a substrate. Root development is controlled by hormones various sets of transcription factors. Recently, small peptides their cognate receptors have been identified as controlling root development. Small bind to membrane-localized receptor-like kinases, inducing dimerization co-receptor proteins for signaling activation givi...

Changes in the visible absorbance spectra of complexes of horse heart cytochrome c hemopeptide 1-65, peptide 66-104, and their guanidinated counterparts are compared with those characteristic of native and fully guanidinated ferricytochrome c over the pH range 7 to 11. Upon raising the pH, the methionine ligand in the guanidinated hemopeptide 1-65.peptide 66-104 complex is replaced by a strong ...

Tetratricopeptide repeat (TPR) domains bind specific peptide ligands and are thought to mediate protein-protein interactions in a variety of biological systems. Here we compare peptide ligand-binding by several different TPR domains. We present specific examples that demonstrate that TPR domains typically undergo little or no structural rearrangement upon ligand binding. Our data suggest that, ...

A thrombin receptor (TR) demonstrating a unique activation mechanism has recently been isolated from a megakaryocytic (Dami) cell line. To further study determinants of peptide ligand-mediated activation phenomenon, we have isolated, cloned, and stably expressed the identical receptor from a human umbilical vein endothelial cell (HUVEC) library. Chinese hamster ovary (CHO) cells expressing a fu...