aqueous two-phase systems (atps) have  emerged as a powerful extraction method for the downstream processing of bio-molecules. the aim of this work was to investigate the possibility of utilizing atps for the separation of recombinant bacillus sphaericus phenylalanine dehydrogenase (phedh). polyethylene glycol (peg) and ammonium sulfate systems were selected for our experiment. the effect of di...

The polymorphic phase behavior of 1-palmitoyl-2-lyso-sn-glycero-3-phosphocholine dispersions in excess water has been studied as a function of temperature and poly(ethylene glycol) (PEG) concentration, using proton dipolar-decoupled 31P NMR spectroscopy and turbidity measurements. The phase behavior was found to depend on both lipid concentration and PEG concentration, and most of the NMR exper...

The effect of coating silica with polyethylene glycol on the adsorption iron and phosphate ions in industrial wastewater was investigated. Variable factors were temperature time coating, PEG concentration, to ratio. Infrared spectroscopy scanning electron microscopy used evaluate chemical structure morphology PEG-coated silica. Optimum removal efficiency obtained using a 50°C, 15 min, concentra...

Aqueous two-phase systems formed by mixing polyethylene glycol (PEG) and dextran (DXT) were studied. The composition of DXT and PEG phase was established by the size exclusion HPLC method. The concentrations of DXT and PEG in both phases as a function of total concentration of both polymers was fitted by nonlinear regression. This procedure yielded an empirical numerical model (with two indepen...

This study aimed to develop an optimized depot injectable atorvastatin (ATR) biodegradable in situ gel (ISG) system with minimum initial burst using a central composite design. The factors selected were poly (d, l-lactide-co-glycolide) (PLGA) concentration (X1), molecular weight of polyethylene glycol (PEG) (X2), and PEG concentration (X3). The independent variables were the initial burst of AT...

Water stress caused by drought affects the productivity of sugarcane crop. A breeding alternative is selection drought-tolerant cultivars. The objective this study was in vitro screening cultivars tolerant to water using polyethylene glycol (PEG) as a stressing agent. Cultivars (cv) Mex 69-290, CP 72-2086, 79-431 and MOTZMex 92-207 were subjected different concentrations PEG 6000 (0, 3, 6 9% w/...

Phase transitions of protein aqueous solutions are important for protein crystallization and biomaterials science in general. One source of thermodynamic complexity in protein solutions and their phase transitions is the required presence of additives such as polyethylene glycol (PEG). To investigate the effects of PEG on the thermodynamic behavior of protein solutions, we report measurements o...

Silicon is known to ameliorate the deleterious effects of drought on plant growth. We evaluated growth of wheat (Triticum aestivum L. CV. Chamran) under Water-Deficit Stress Induced by Polyethylene Glycol as affected by Si application. In this article, the effects of Si (as potassium silicate) on some parameters related to growth, chlorophyll concentration relative water content (RWC), electrol...

The regulation of cell motility on ligand-adsorbed poly(ethylene glycol) (PEG)-based polymeric biomaterials is governed by variables that are not well characterized. In this report, we examined keratinocyte migratory responsiveness to PEG-variant tyrosine-derived polycarbonates adsorbed with equivalent levels of the cell adhesion ligand, fibronectin. The equivalently adsorbed ligand adopted dif...