Leishmania (Leishmania) infantum is the main etiologic agent of visceral leishmaniasis in American continent. We report a rare case of cutaneous leishmaniasis caused by viscerotropic specie in a patient with ankylosing spondylitis on treatment with adalimumabe and methotrexate. The patient presented no signs of visceral involvement. PCRRFLP and genetic sequencing demonstrated Leishmania (Leishm...

The present work was carried out to analyse the κ-casein variants in five Indian goat breeds by SDS-PAGE, PCRRFLP and SSCP method. A total of 152 unrelated blood samples and 102 milk samples belonging to Barbari, Beetal, Marwari, Surti and Local MP (non-descript) goats were used for analysis. SDS-PAGE exhibited κ-casein allele A in all the breeds. Moreover PCR+RFLP analysis also confirmed the p...

MatrixMetalloProteinases-2, are zinc dependent endopeptidases which degrade major components of the basal lamina around blood vessels. Myocardial Infarction is mainly due to atherosclerosis of the coronary arteries. MMP-2 destabilizes the arterial wall and breaks atheromatous plaque leading to MI. The gene for human MMP-2 contains 27,862 base pair genomic DNA and is composed of 13 exons. Severa...

Maternal zinc deficiency seems to be a risk factor for orofacial clefts in offspring. This study was undertaken to investigate the involvement of polymorphic variants of genes for zinc transporters in the susceptibility of clefting. PCRRFLP analysis was used to analyze single nucleotide polymorphisms of SLC30A1 (rs7526700, rs2278651, rs611386), SLC30A4 (rs2453531, rs8029246), SLC30A5 (rs351444,...

A method for the identification of three ixodid tick species; the cow tick (Boophilus annulatus), the camel tick (Hyalomma dromedarii) and the cattle tick (Hyalomma anatolicum excavatum) based on restriction enzyme analysis of the second internal transcribed spacer (ITS-2) in the nuclear ribosomal DNA was adopted. PCR products with MW 1375 bp, 1650 bp and 1835 bp from B. annulatus, H. dromedari...

background: vancomycin (glycopeptide)-resistant enterococci (vre or gre) can cause serious problems for hospitalized patients due to the limited options for treatment of vre infections. as infection with vre increases in hospitals, further knowledge about vancomycin resistant genes is needed. methods: isolates of enterococcus spp. were collected from hospitalized patients in tehran (iran) durin...

Background: Vancomycin (glycopeptide)-resistant enterococci (VRE or GRE) can cause serious problems for hospitalized patients due to the limited options for treatment of VRE infections. As infection with VRE increases in hospitals, further knowledge about vancomycin resistant genes is needed. Methods: Isolates of Enterococcus spp. were collected from hospitalized patients in Tehran (Iran) durin...