Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated endonuclease Cas9 (Cas9) has high specificity to its target DNA as a gene editing tool. This characteristic makes it useful for detection. Combining the advantages of CRISPR/Cas9 and PCR, this study establishes novel CRISPR/Cas9-based detection method, named CRISPR/Cas9-typing PCR version 4.0 (ctPCR4.0). method...