Edwardsiella tarda is an enterobacterial fish pathogen that causes mortality in various fish species worldwide. In this study, we analyzed the intraspecific variability in a collection of E. tarda strains isolated from turbot. To do this we employed 4 polymerase chain reaction (PCR)-based methods: (1) random amplified polymorphic DNA (RAPD), (2) enterobacterial repetitive intergenic consensus-P...

Random-amplified-polymorphic-DNA(RAPD) was assayed to detect the genetic variation of 6 barley generations from Iraq. Four primers generated a total 17 scoreable bands in RAPD analysis) and resolving power, three polymorphic differed (Rp). The use marker systems distance among generation discovered be beneficial. dendrograms indicate diverse grouping barely specimens, although we did see that c...

We described the use of the random amplified polymorphic DNA (RAPD) technique on Plasmodium falciparum DNA to detect genetic markers for chloroquine-resistant strains. Fourteen RAPD primers were tested, three of which generated banding patterns correlated with chloroquine resistance. To measure this correlation, the RAPD profiles were analyzed using the Nei and Li similarity coefficient. Detect...

An attempt was made to evaluate the utility of a method which employs semi-specific PCR using partially specific primers for the coding sequence (ET) at the exon-intron contact and of the RAPD method to identify eight Polish cultivars of gerbera. It was demonstrated that the PCR method which employs semi-specific primers is as simple and economical as the RAPD method, simultaneously the images ...

DNA fingerprinting methods were used to follow the progress of unmarked starter cultures in laboratory sauerkraut fermentations (1.2 and 13 l). Random prime PCR (RAPD-PCR) was used for strain-specific identification of Leuconostoc mesenteroides cultures. A comparative analysis of RAPD banding patterns for fermentation isolates and starter cultures was carried out using both genetically marked a...

Wine quality is determined by the particular yeast strains prevailing at various stages of fermentation. Therefore, ability to make an easy, fast, and unambiguous discrimination yeasts strain level great importance. Here, tandem repeat-tRNA (TRtRNA) method with 5GAC or ISSR-MB primer sets random amplified polymorphic DNA (RAPD) analysis (GTG)3, R5, RF2 oligonucleotides were tested on non-Saccha...

Molecular markers are used in plant science a variety of ways. The method molecular genome marking based on RAPD-PCR makes it possible to determine the genetic status populations and establish intrapopulation relationships. In this work, we studied polymorphism dandelion Taraxacum officionale using RAPD markers. Five random amplified polymorphic DNA (RAPD) were diversity relationship between tw...

The combined use of the arbitrarily primed polymerase chain reaction [AP-PCR, also known as random amplification of polymorphic DNA (RAPD}] and isozyme mapping resulted in the production of 87 potential marker loci, enabling an overall expansion within the genetic map of the fish genus Xiphophorus. Use of DNA sequencing-style acrylamide gels and carefully controlled conditions of amplification ...

The use of random amplified polymorphic DNA from the polymerase chain reaction (RAPD-PCR) allows efficient construction of saturated linkage maps. However, when analyzed by agarose gel electrophoresis, most RAPD-PCR markers segregate as dominant alleles, reducing the amount of linkage information obtained. We describe the use of single strand conformation polymorphism (SSCP) analysis of RAPD ma...