BACKGROUND Mycoplasma pneumoniae is a common cause of community-acquired pneumonia (CAP) in children. The aim of this study was to assess the prevalence of Mycoplasma pneumoniae infection in children with CAP and find clinical, radiological and laboratory features helpful to diagnose Mycoplasma pneumoniae pneumonia. Furthermore, we evaluated the value of serology, real-time PCR (RT-PCR) and cul...

Mastitis is one of the main diseases that affects dairy cattle. It characterized by a multifactorial disease with multiple etiologies including microorganisms such as bacteria, viruses, fungi, yeasts, and algae. caused Mycoplasma spp. results in contagious nature infection has been studied much Brazil. The objectives this research were (1) to investigate clinical mastitis milk samples, conventi...

Mycoplasma pulmonis and Mycoplasma arthritidis were differentially identified using PCR-restriction fragment length polymorphism (RFLP). A genus-specific sequence of mycoplasma was amplified by PCR and the PCR products were digested with the restriction enzyme SmaI. Each PCR product from the four isolates of M. pulmonis was digested with SmaI into two fragments; however, there was no digestion ...

mycoplasma gallisepticum is the most important and infectious mycoplasmosis. it is caused lots of economic losses for poultry's industry of iran. the target of this study is comparison of culture and nested pcr techniques to detect mycoplasma gallisepticum infection of chicken’s from industrial farms in kerman province of iran. 88 isolates received from industrial farms of kerman province of ir...

mycoplasma synoviae (ms) is an important avian pathogen that can cause both respiratory disease and joint inflammation synovitis in poultry, inducing economic losses to the iranian chicken industry especially breeder farms. the aim of this study was to use the ms specific pcr and culture methods in order to detect of m. synoviae from breeder farms where located in tehran province. a total of 47...

In the present study, serologic data were compared with data obtained by capillary PCR to establish the efficacy of capillary PCR for the determination of Mycoplasma infection in samples obtained from throat swabs, bronchoalveolar lavage fluids (BALF), and sputum of patients with Mycoplasma pneumonia. We performed PCR analysis for Mycoplasma DNA on a total of 325 samples from 197 patients with ...

Mycoplasma 352 Molecular characterization of mycoplasma gallisepticum isolated from chicken and turkey. 353 Pathogenicity of Mycoplasma gallisepticum strains using ELD50 in embryonated chicken eggs. 354 Evaluation of three DNA extraction methods for the detection of Mycoplasma spp. with an MG/MS multiplex real-time PCR method. 356 Use of MG/MS serology and PCR to determine flock status. 357 Exp...

Contamination of cell cultures and sera used for animal virus propagation with mycoplasmas represents a serious problem, especially in virology. Therefore specific control measures must be used. To achieve this we introduced PCR for the detection of mycoplasma species in cell cultures and compared its results with ELISA and microbiological culture. Seven mycoplasma species which are the most co...

The dromedary camels are essential because of their multiple uses for transport, wool, meat, and milk production. Camel’s is considered exceptional nutrition, so it therapeutic high nutrition. Mastitis may affect camel’s production, cause significant economic loss, associated with zoonotic disease. study aimed to detect the prevalence Mycoplasma spp. especially M. bovis in using polymerase chai...