نتایج جستجو برای: pcr hemi

تعداد نتایج: 177725  

Journal: :The new microbiologica 2005
Duà Nazzal Salem Yasin Khaled Abu-Elteen

The rapid detection and identification of Candida species in clinical laboratories are extremely important for the management of patients with hematogenous candidosis. Currently available culture and biochemical methods for detection and identification of Candida species are time-consuming. This study describes the use of a simple and rapid PCR method using species-specific oligonucleotides for...

Amirhosein Maali, Hamid Gholipour, Mehdi Azad, Mehrdad Noruzinia, Saeid Abroun, Sasan Ghaffari,

Background: Epigenetic modifications, such as methylation can occur in multiple myeloma. SMG1is an important gene involved in cell growth which defect in methylation of its promoter leads to reduction of cell apoptosis and uncontrolled proliferation. In this study, we identified the methylation status of the SMG1 gene promoter in patients with multiple myeloma. Methods: Methylation status of S...

2009
J Coertse L. H. Nel W Markotter

The genus Lyssavirus is divided into 7 genotypes. Genotype 1 is found worldwide and genotypes 2-4 are found exclusively on the African continent. Representatives of genotypes 1-4 were selected to include every lineage, group and biotype within these genotypes to account for the huge diversity observed in this genus. A reverse transcription PCR as well as a hemi-nested PCR method has been develo...

Journal: :Genetics and molecular research : GMR 2012
J V Luz A S Alcântara-Neto R I T P Batista J M G Souza D I A Teixeira L M Melo V J F Freitas

The CD44 family belongs to a larger group of hyaluronic acid-binding proteins and plays important roles in oocyte maturation, fertilization and preimplantational embryo development. We analyzed the CD44 receptor in sheep oocytes and embryos. Immature oocytes (N = 66) were obtained from a local abattoir; mature oocytes (N = 35) and embryos (N = 41) were obtained by laparotomy from adult hai...

2017
Ajaz Ahmad C. K. Singh

Twenty seven dog brain tissues were examined for rabies infection by fluorescent antibody technique, immunohistochemistry and Hemi-Nested Reverse Transcriptase (HnRT-PCR). 16 (59.25%) dogs were confirmed to be rabid by FAT and HnRT-PCR assay while 15 samples were positive by immunohistochemistry (IHC). Sensitivities of HnRT-PCR and IHC were 100% and 94.11% respectively whereas both approaches d...

دکتر تقی طریحی, , دکتر سید علیرضا مصباح, , فرزانه رهمدانی, ,

  چکید ه   سابقه و هدف   آنتی‌ژن RhD در ایجاد بیماری‌های همولیتیک نوزادان نقش مهمی دارد و از آن‌جایی که تعیین RhD جنین با استفاده از روش‌های تهاجمی مانند آمنیوسنتز، خطراتی را برای مادر و جنین به دنبال خواهد داشت، در این پژوهش تلاش گردید تا ژنوتایپ RhD جنین از DNA آزاد جنینی موجود در سرم مادران باردار، به صورت غیر تهاجمی و با استفاده از روش Hemi-nested PCR تعیین گردد.   مواد وروش‌ها   مطالعه انج...

Journal: :The American journal of tropical medicine and hygiene 2008
Hilary Wolf Melissa Mendez Robert H Gilman Patricia Sheen Giselle Soto Angie K Velarde Mirko Zimic A Roderick Escombe Sonia Montenegro Richard A Oberhelman Carlton A Evans

Pediatric pulmonary tuberculosis diagnosis is difficult because young children are unable to expectorate sputum samples. Testing stool for tuberculosis DNA from swallowed sputum may diagnose pulmonary tuberculosis. Hospitalized children with suspected tuberculosis had stool, nasopharyngeal, and gastric aspirates cultured that confirmed pulmonary tuberculosis in 16/236 patients. Twenty-eight sto...

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