PCR-based DNA fingerprinting was used to characterize 48 clinical isolates of Yersinia enterocolitica. The samples were examined by random amplified polymorphic DNA (RAPD-PCR) and inter-repeat PCR (IR-PCR). IR-PCR with two enterobacterial repetitive intergenic consensus primers resulted in patterns which were poorly discriminated; 2 of 11 arbitrary primers (RAPD-PCR) provided sufficient discrim...

In conventional DNA fingerprinting, hypervariable and repetitive sequences (minisatellite or microsatellite DNA) are detected with hybridization probes. As demonstrated here, these probes can be used as single primers in the polymerase chain reaction (PCR) to generate individual fingerprints. Several conventional DNA fingerprinting probes were used to prime the PCR, yielding distinctive, hyperv...

objective(s) pseudomonas aeruginosa is the most important cause of chronic lung infections and death in patients with cystic fibrosis. determining the distribution of specific strains within patient populations is important in order to examine the epidemiology of the disease and the possibility of cross infection among patients. materials and methods forty six iranian patients with cystic fibro...

Characterization of 43 strains of Rhizobium leguminosarum biovars viciae, trifolii, and phaseoli was performed by two methodologies based on PCR amplification, i.e., PCR DNA fingerprinting of interrepeat sequences and restriction fragment length polymorphism (RFLP) analysis of PCR -amplified chromosomal and symbiotic gene regions. Groupings generated by PCR DNA fingerprinting with either extrag...

Phenotypic approaches based on metabolic and biological characteristics of Corynebacterium pseudotuberculosis have been limited due to insufficient discrimination between closely related isolates. In this paper we present performance and convenience of three molecular typing methods: BOX-PCR, random amplification of polymorphic DNA (RAPD) and amplification of DNA fragments surrounding rare rest...

We expanded the basic ISSR-PCR protocol by an additional PCR reamplification round in order to detect whether increased PCR productivity would give new bands in ISSR patterns. We found that the reamplification step had a prominent impact on the quality of the inter-simple-sequence repeat (ISSR) PCR patterns of flax, depending on the particular primer used for PCR amplification. We could clearly...

evaluation of genetic diversity is a considerable approach to screen within indigenous rhizobial population for compatible, highly effective strains, which can be further utilized as rhizobial inoculums. genetic diversity of rhizobium isolates from three clover species grown in soil samples of seven different geographical regions in iran was investigated by repetitive extra genomic palindromic ...

background: colibacillosis is one of the most economically important diseases of poultry worldwide. objectives: this study was conducted to examine the clonal relatedness and typing of 95 avian escherichia coli isolates by eric-pcr. methods: sixty-three e. coli isolates from two common manifestations of colibacillosis (yolk sac infection and colisepticemia) and 32 isolates from feces of apparen...