background and objectives: tuberculosis (tb) is the oldest known bacterial disease in humans. due to the rise of morbidity in recent years, early diagnosis of the disease is necessary. materials and methods: in this study we used fluorescent amplification–based specific hybridization (flash) pcr to target is6110 for rapid detection of m. tuberculosis (mtb). to investigate the important factors ...

a multiplex pcr-based assay was developed for detection of salmonella typhi and identification of other salmonella serotypes. three primer-sets were selected from different genomic sequences, malo2-f/malo2-ra primers from invasion gene, parat-s/parat-as as well as tyv-s/tyv-as primers from o-antigen gene cluster of the genus salmonella. this method differentiated salmonella spp., based on size ...

background: neisseria meninigitidis is one of the most frequently encountered microorganisms associated with central nervous system infections. the aim of this study was to evaluate a pcr-based assay for specific and rapid detection of n. meninigitidis in csf specimens.   methods : since april 2002 to july 2006, 130 csf specimens were collected from patients suspected of having bacterial mening...

objectives: salmonella typhimurium is important food-borne pathogen responsible for gastroenteritis. in this work a polymerase chain reaction based enzyme linked immunosorbent assay (pcr-elisa) was developed to identify salmonella typhimurium. materials and methods: the rfb gene which is responsible for biosynthesis of the salmonella o-antigenic lipopolysaccharide was selected as the target s...

background: salmonella enterica serovar typhi, as causative agent of typhoid fever, is one of the most important endemic pathogens. non-typhoidal salmonella serovars, including typhimurium , infantis , and enteritidis are amongst the most prevalent serotypes worldwide and in developing areas such as iran. the aim of this study was to apply a uniplex pcr for rapid detection of salmonella spp., a...

background and objectives: contamination of cell lines and biological products is one of the major problems of cell culture techniques. rapid detection of mycoplasma contamination in cell culture is an important part of quality control standards in related laboratories. the aim of this study was to evaluate the efficacy of pcr in detection of myroplasma as contaminants in cell cultures and othe...

objective: bacterial meningitis is a dangerous and sometimes fatal infection that affects the central nervous system. because some antibiotics can prevent some types of these bacteria and supress them from spreading and infecting, therefore it is important to know what type of virus or bacterium is causing meningitis. haemophilus influenzae and neisseria meningitides are the two main pathogens...

the present study was undertaken to screen the soil samples collected in iran for the presence of the bacillus subtilis lipase a gene. the bacterial colonies obtained from the collected soil samples were examined by physical appearance, biochemical tests and  the polymerase chain reaction (pcr). only four colonies were identified as putative b. subtilis strains and all contained the lipase a ge...

background escherichia coli o157:h7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. methods we developed fluorescent amplification-based specific hybridization (flash–pcr) assay to detect the stx-encoding gene stx-1 of e. coli o157:h7. result pcr product of 336 bp was suc...