ES cells. This phenomenon has been observed in our laboratory in a number of cases. Clones putatively identified as homologous recombinants by Southern analysis can be quickly checked for random insertion of extra copies of the targeting vector by PCR. Given the small difference in size between the two alleles and use of only two primers common to both WT and mutant DNA, the amplification rate ...

Abstract Objective We investigated the discrepancy between clinical and PCR-based diagnosis of COVID-19. compared results ten patients with mild to severe Respiratory samples from all cases were tested on Roche SARS-CoV-2 (Cobas) assay, Filmarray RP2.1 (bioMereiux) TaqPath™ COVID19 (Thermofisher) PCR assays. Results Laboratory records COVID-19 examined. Initially, respiratory as negative ® assa...

HIGHLIGHTS A multiplex PCR to identifying mycobacteria of medical interest was evaluated. Mycobacterium tuberculosis can be differentiated from other with the assay. The assay showed high sensitivity and specificity in detecting M. avium complex.