INTRODUCTION Tooth extraction to provide sufficient space, or camouflage of underlying skeletal problems is quite common in orthodontics. The present study evaluated soft tissue changes after upper first premolars extraction in class ΙΙ div 1 patients. MATERIAL AND METHODS 20 cases (15 females, 5 males), with a mean age of 17.8±2.9 years with class ΙΙ div1 malocclusion and normal vertical hei...

The phoN gene of Salmonella enterica sv. Typhimurium strain MD6001 was cloned in the multicopy plasmid pBluescript SK(-). The nucleotide sequence of the cloned gene differs from the corresponding S. typhimurium LT2 sequence at 23 residues, leading to 15 amino-acid differences, but was very close to the S. typhi phoN sequence (only three nucleotide and two amino-acid differences). The recombinan...

Plasmid vectors based on pMB8, such as pBR322, pUC 18, pGEM5 and pBluescript (1), have become virtually indispensable tools in molecular biology. Unfortunately, pMB8 derived vectors are restricted to Entembacteriacea. Transfer of cloned DNA fragments into other bacterial species therefore necessitates alternative plasmid vectors. Insertion of the gene of interest into such (broad-host-range) pl...

Plasmid pBG 35 contains the whole rDNA unit from flax (Linum usitatissimum L.) (Goldsbrough and Cullis, 1981). We cloned four different Eco RI restriction rDNA fragments of pBG 35 in the plasmid vector pBluescript SK+ (pBS). One of the subclones (p2.1) contained the most part of the 5 ́-end of 18S rRNA gene (1581 bp) and was used as a heterologous DNA probe in molecular hybridization experiments...

A carboxymethyl cellulase gene, cel5B, was cloned, sequenced, and expressed in Escherichia coli. pRCS20 in E. coli was identified from metagenomic cosmid library of cow rumen for cellulase activity on a carboxymethyl cellulose agar plates. Cosmid clone (RCS20) was partially digested with Sau3AI, ligated into BamHI site of pBluescript II SK+ vector, and transformed into E. coli DH5α. The insert ...

Abstract Background DEXA scan could be unavailable at some health centers, and radiologic examination of the mandible oral cavity is considered more commonly used test that can predict, diagnose, or even follow-up on any defect in bone mineralization. The aim this study was to elucidate ability panoramic radiograph detect osteoporosis rheumatoid arthritis patients correlate mandibular index wit...

Single-crossover homologous integration in Lactobacillus sake was studied. Integration was conducted with nonreplicative delivery vector pRV300. This vector is composed of a pBluescript SK- replicon for propagation in Escherichia coli and an erythromycin resistance marker. Random chromosomal DNA fragments of L. sake 23K ranging between 0.3 and 3.4 kb were inserted into pRV300. The resulting pla...

By making use of preparative field inversion gel electrophoresis, we have constructed a lambda ZAP library that is highly enriched for sequences from the choroideremia locus. In vivo excision of pBluescript SK(-) constructs from lambda ZAP obviates the subcloning of DNA inserts and allows for rapid processing of several hundred recombinants. From a 625 kb Sfil fragment we isolated 7 clones that...

The binding of proteins to specific DNA sequences frequently results in localized bending of the DNA, which may play important roles in regulating transcription of a variety of genes (1,3). The pBend family of plasmids (2,4) allow generation of permuted fragments containing cloned DNA-binding sites and provide a convenient and widely used method for analyzing DNA bending. In most popular clonin...