The potato tuber moth (Phthorimaea operculella Zeller) (PTM) is a destructive pest. Bacillus thuringiensis cry genes encode crystal proteins that have toxic characteristics against PTM. aim of the present study was to develop PTM resistant cv. Agria using synthetic Bt cry1Ab gene. transformation vector constructed by inserting gene into pBI121 binary expression vector. Agrobacterium tumefaciens...

The main goal of this study was to investigate the possibility of expressing recombinant protein of C-C chemokine ligand 21 (CCL21) in Solanum lycopersicum via agroinfiltration. CCL21 is a chemokine can be used for anti-metastatic of cancer cell lines. To examine the expression of CCL21 protein in S. lycopersicum, the construct of ccl21 was synthesized. This construct was cloned into pBI121 and...

Cell transformation of Arabidopsis thaliana (L.) Heynh. with Agrobacterium tumefaciens (LBA4404 strain) was performed in two ways: in vitro by the transformation of root and stem explants and inplanta by the transformation of whole plants in the green­ house. Agrobacterium contained the binary plasmid pBI121 or its constructs with the fragments of carrot DC-8 promoter. The results show that tra...

The bovine viral diarrhea virus (BVDV), a single-stranded RNA virus, can cause fatal diarrhea syndrome, respiratory problems, and reproductive disorders in herds. Over the past few years, it has become clear that the BVDV infection rates are increasing and it is likely that an effective vaccine for BVDV will be needed. In this study, transgenic Astragalus was used as an alternative productive p...

The Agrobacterium-mediated transformation of hairy roots creates a rapid and simple means to introduce and express foreign genes in plant cells that are capable of synthesizing specific natural products. We describe a method for the production of transgenic hairy root cultures of peanut (Arachis hypogaea L.) using Agrobacterium rhizogenes R1000, a strain with the binary vector pBI121. We evalua...