OBJECTIVE To compare directly in vitro developmental competence between parthenogenetically activated and intracytoplasmic sperm injection (ICSI)-fertilized oocytes. DESIGN For each patient, three metaphase II oocytes were randomized to the ICSI procedure, while n-3 were allocated to parthenogenetic activation. SETTING University hospital infertility unit. PATIENTS Thirty-eight patients, ...

This study determines the efficiency of sequential calcium treatments (electroporation or ionomycin) combined with protein synthesis (cycloheximide) or phosphorylation inhibitors (6-dimethylaminopurine) or the specific maturation promoting factor (MPF) inhibitor, roscovitine, in inducing artificial activation and development of rhesus macaque parthenotes or nuclear transfer embryos. Exposure of...

OBJECTIVE To explore the ability of diacyl glycerol (DAG) and inositol triphosphate (IP3), two major secondary messengers in the calcium signaling pathway, in activating oocytes. MATERIAL AND METHODS Oocyte cumulus complex obtained from superovulated Swiss albino mice were incubated in M16 medium with liposome-encapsulated 1,2-Dipalmitoyl-sn-glycerol (LEDAG) and/or IP3 for 3 h. Strontium chlo...

In this study, a dose-response assessment was performed to understand the relation between supplementation of media with L-ascorbic acid or vitamin C and porcine oocyte maturation and the in vitro development of parthenotes (PA) and handmade cloned (HMC) embryos. Various concentrations (0, 25, 50 and 100 μg/ml) of vitamin C supplemented in in vitro maturation (IVM) and culture (IVC) media were ...

The incidence of parthenogenetic development of oocytes in routine IVF practices i.e. without any chemical stimuli, was assessed. After 24 h of maturation while washing and transferring to fertilization medium nine (1.39%) oocytes out of 646 oocytes were found cleaved. All those parthenotes (cleaved oocytes) were transferred to embryo culture medium instead of fertilization medium for observing...