To identify the genetic events which may play a role in the development of cervical carcinoma, we performed a detailed allelotype analysis utilizing DNA from 53 primary tumors and corresponding normal cells and 57 polymorphic probes mapped to each of the chromosomal arms, excluding the short arms of the acrocentric chromosomes. Loss of heterozygosity (LOH) of >25% was observed at sites on 11 ch...

This study was undertaken to develop technology for the detection of nucleic acid using two different DNA probe reporter molecules, the ultimate aim being to differentially label two nucleic acids within the same nucleus. Digoxigenin and biotin were used to label DNA probes. The absolute and relative sensitivity of digoxigenin and biotin labelled DNA probes for detecting integrated human papill...

Human papilloma virus (HPV) was isolated from red plaques of a patient (N. F.) with epidermodysplasia verruciformis. Elec tron microscopic examination showed characteristic particles of papilloma virus as ¡cosahedrons about 45 nm in diameter. DNA was extracted from these particles, and closed-circular DNA (Form I) was purified by centrifugation in CsCI containing ethidium bromide. The molecula...

Professor Harald zur Hausen, emeritus Scientific Director and Chairman of the Management Board of the German Cancer Research Center in Heidelberg, has made seminal observations that identify novel human papilloma viruses as key contributors to cervical cancer. Cervical cancer is the second most common cancer among women. Professor zur Hausen's discoveries include detection of novel human papill...

Human papilloma virus (HPV) was isolated from red plaques of a patient (N. F.) with epidermodysplasia verruciformis. Electron microscopic examination showed characteristic particles of papilloma virus as icosahedrons about 45 nm in diameter. DNA was extracted from these particles, and closed-circular DNA (Form I) was purified by centrifugation in CsCl containing ethidium bromide. The molecular ...

Background and Aims: Ovarian cancer is one of most common causes of cancer related women's mortalities. Human papilloma virus is a known factor concerning cervical cancer but its role in causing ovarian cancer is not yet verified. A few studies also identified HPV DNA in ovarian carcinoma tissues. However, some studies did not detect HPV DNA in ovarian carcinoma tissues. In this articl...

The aim of this study was to reveal whether 3 biomarkers (p16INK4a, ProEx C, and human papilloma virus DNA) are useful in the diagnosis of cervical intraepithelial neoplasia and whether they could predict disease progression of cervical intraepithelial neoplasia-1. We analyzed 252 cervical specimens: nondysplastic mucosa (n = 9), cervical intraepithelial neoplasia (n = 229), and squamous cell c...

A case of Bowen's disease arising on the medial part of the first metatarsal bone of an 81-year-old Japanese woman is described. Histopathologically, proliferation of atypical cells was found throughout the epidermis. Electronmicroscopy revealed virus particles 40-50 nm in diameter in the nuclei of tumour cells at the granular cells just on or below the horny layer. Positive bands were obtained...

background and aims: ovarian cancer is one of most common causes of cancer related women's mortalities. human papilloma virus is a known factor concerning cervical cancer but its role in causing ovarian cancer is not yet verified. a few studies also identified hpv dna in ovarian carcinoma tissues. however, some studies did not detect hpv dna in ovarian carcinoma tissues. in this article, we inv...