In 2001, 698 urine samples were randomly collected from cattle at a slaughterhouse in the State of Paraná, Brazil. Direct examination using dark field microscopy was carried out immediately after collection. Five putative positive samples were cultured in modified EMJH medium, yielding two positive cultures (LO-14 and LO-10). Typing with monoclonal antibodies revealed that the two isolates were...

In order to investigate the seroprevalence of leptospirosis in traditional dairy farms, a serological study was conducted in Gilan province. For this study,, a total of 205 serum samples were randomly collected from nine districts of this province. All serum samples were serologically tested by the standard MAT using live antigens representing the following Leptospira interrogans serovars: Grip...

Stalheim, O. H. V. (University of Wisconsin, Madison), and J. B. Wilson. Cultivation of leptospirae. I. Nutrition of Leptospira canicola. J. Bacteriol. 88:48-54. 1964.-The nutrition of Leptospira canicola was investigated by use of synthetic media of suitable ionic strength. At an incubation temperature of 30 C, the minimal components were calcium, iron, magnesium, and ammonium ions, thiamine, ...

Leptospirosis is a zoonosis of worldwide distribution, caused by Leptospira interrogans. It is a well known cause of bovine reproductive losses such as abortion, infertility, stillbirth and birth of weak calves. Inthis research, the relationship between the seroprevalence rate of Leptospira spp. infection and abortion inindustrial dairy farms of Hamedan province, Iran was studied. A total of 80...

This research aims at studying sero infection of leptospira interrogans in farms of Sarab. For this purpose, 200 serum samples were randomly collected. Six kinds of alive leptospira antigens, ballum, canicola, grippotyphosa, icterohaemorrhagia, hardjo,Pomona were isolated using MAT test. The results obtained from this test demonstrated that 23% of animals sera were serologically positive for di...

department for agglutination reaction for leptospiral infection. The serum was put up against all the strains of leptospira then available in the laboratory, viz, strain Chopra (classical L. icterohcemorrhagice), Mg. Tin Tin (= Java bat strain), Andaman CH 31, Andaman CH 11, and L. canicola. It failed to agglutinate all these strains except L. canicola. In the latter a few definite clumps were ...