The herpes simplex virus type 1 (HSV-1) immediate-early gene IE63 (ICP27), the only HSV-1 regulatory gene with a homologue in every mammalian and avian herpesvirus sequenced so far, is a multifunctional protein which regulates transcriptional and posttranscriptional processes. One of its posttranscriptional effects is the inhibition of splicing of viral and cellular transcripts. We previously i...

Pax-6 is an evolutionarily conserved transcription factor regulating brain and eye development. Four Pax-6 isoforms have been reported previously. Although the longer Pax-6 isoforms (p46 and p48) bear two DNA-binding domains, the paired domain (PD) and the homeodomain (HD), the shorter Pax-6 isoform p32 contains only the HD for DNA binding. Although a third domain, the proline-, serine- and thr...

p32/gC1qR is a small acidic protein that has been reported to have a broad range of distinct functions and to associate with a wide array of cellular, viral and bacterial proteins. It has been found in each of the main cellular compartments including mitochondria, nucleus and cytoplasm and is also thought to be located at the plasma membrane and secreted into the extracellular matrix. The true ...

1. When enzyme secretion was stimulated by carbamylcholine or acetylcholine (with eserine) in slices of pigeon pancreas, the incorporation of P32 into the phospholipide fraction of the stimulated slices was, after 2 hours, 4.8 to 8.7 (average, 7.0) times greater than the incorporation of P32 into the phospholipides of control slices. Neither respiration nor the incorporation of P32 into acid-so...

The synthesis of a Mr 32,000 protein (p32) is enhanced as early as 2 h after the addition of tumor-promoting phorbol esters to BALB/c 3T3 cells. Among various compounds tested thus far, methyl methanesulfonate, N-methyl-N'-nitro-N-nitrosoguanidine, L-ascorbic acid, sodium deoxycholate, p-tosyl-L-phenylalanine chloromethyl ketone, p-tosyl-L-lysine chloromethyl ketone, 3,3'-diaminobenzidine, and ...

Rabies, caused by rabies virus (RABV), is an acute, fatal encephalitic disease that affects many warm-blooded mammals. Currently, post-exposure prophylaxis regimens are effective for most rabies cases, but once the clinical signs of the disease appear, current treatment options become ineffective. Carrageenan has been reported as a potent inhibitor of many viruses. In this study, the λ-carragee...

The effect of 12-O-tetradecanoylphorbol 13-acetate (TPA), a potent tumor promoter, on the synthesis of proteins in BALB/c 3T3 mouse fibroblasts has been studied. When [35S]methionine-labeled proteins synthesized after the addition of TPA were analyzed by one- or two-dimensional polyacrylamide gel electrophoresis, the increased synthesis of a 32,000-dalton protein (designated p32) was noted as o...

The synthesis of a protein of M, 32,000 (p32) is enhanced by various tumor promoters, chemical carcinogens, metal salts, and heat shock in BALB/c 3T3 cells. We have isolated a complementary DNA (cDNA) clone for p32 from a XgtlO library of BALB/c 3T3 cells. The library was constructed from mRNA extracted from the cells treated with sodium arsenite, which stimulates the p32 expression most effect...

32 To clarify the function(s) of the herpes simplex virus 1 (HSV-1) major virion 33 structural protein UL47 (also designated VP13/14), we screened cells overexpressing UL47 34 for UL47-binding cellular proteins. Tandem affinity purification of transiently expressed 35 UL47 coupled with mass spectrometry-based proteomics technology and subsequent analyses 36 showed that UL47 interacted with cell...

The synthesis of a unique protein with a molecular weight of 32,000 (p32) in BALB/c 3T3 cells has been shown previously to increase after treatment with potent tumor-promoting phorbol esters (Hiwasa et al., Proc. Natl. Acad. Sci. U. S. A., 79: 1800, 1982). In the present study, two new classes of tumor promoters which are structurally different from phorbol esters were investigated for their po...