نتایج جستجو برای: ovine embryo
تعداد نتایج: 75354 فیلتر نتایج به سال:
BACKGROUND The impact of prion proteins in the rules that dictate biological reproduction is still poorly understood. Likewise, the role of prnt gene, encoding the prion-like protein testis specific (Prt), in ram reproductive physiology remains largely unknown. In this study, we assessed the effect of Prt in ovine fertilization by using an anti-Prt antibody (APPA) in fertilization medium incuba...
in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.
The aim of this study was to test the accuracy of genotype diagnosis after pre-amplification of DNA extracted from biopsies obtained by microblade cutting of ovine embryos and to evaluate the viability of biopsied embryos after vitrification/warming and transfer to recipients. Sex and PrP genotypes were determined. Sex diagnosis was done by PCR amplification of ZFX/ZFY and SRY sequences after P...
Heat shock may affect different aspects of oocyte maturation and its subsequent development to the blastocyst stage. A series of in vitro experiments was performed to determine whether physiologically heat shock (41°C) disrupts the progression of the ovine oocytes through meiosis, activation and blastocyst formation. Cumulus-oocyte complexes (COCs) were aspirated from 2-6-mm follicles and cultu...
Twenty-five crossbred ewes were utilized to examine the timing of embryonic migration relative to maternal recognition of pregnancy. These ewes also were utilized to examine whether ovine embryos synthesized estradiol-17 beta in association with embryonic elongation and intrauterine migration. Embryos were flushed on d 11 through 15 from hemiovariectomized ewes. Recovery of embryos from the ute...
BACKGROUND Linoleic acid (LA) is a polyunsaturated fatty acid present in high concentrations in follicular fluid, when added to maturation culture media, it affects oocyte competence. OBJECTIVE In the present study, we investigated effect of linoleic acid supplementation on in vitro maturation, embryo development and apoptotic related gene expression in ovine. MATERIALS AND METHODS The expe...
Background: Oocyte in vitro maturation success rate is still low in compareision to oocyte in vivo maturation. Thus prediction and improvement of oocyte competency are two critical issues in assisted reproductive technology. New insights into oocyte-cumulus cell regulatory loop may assist our optimization of in vitro maturation technique. Maturation and developmental competency of cumulus oocyt...
In order to evaluate the effect of O2 tension on the in vitro maturation (IVM) and fertilization (IVF) process, 11 replicates of OPU/IVP were performed using 1092 viable oocytes obtained from 48 Bos indicus embryo donors of commercial herds. Cumulus-oocyte complexes (COCs) from each donor were homogeneously and randomly distributed across two treatments. The IVM was performed in TCM-199 with ov...
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