The HCl-extracted purified polysaccharide type antigens of group B Streptococcus types Ia and Ib were composed of galactose and N-acetylglucosamine in a molar ratio of 3:1 for the Ia antigen and 2:1 for the Ib antigen. Immunochemical data were the same for the Ia antigens of type Ia, purified in this study, and type Ic, purified earlier. Glucosamine inhibited the Ib quantitative precipitin reac...

one way to confront chemical warfare in order to reduce their harmful effects, is to use the body's defense mechanisms. in order to do so firstly mustard gas (hd) was injected in an animal model in the form of an immunogen and the induced humoral immune response was investigated by two different immunological methods such as the ouchterlony test and a new liquid phase fluoroimmunoassay. 7l...

Myeloma proteins (1) and antibodies of a given specificity (2, 3) produced in an individual animal possess individually specific or "idiotypic" (4) antigenic determinants. 1 Idiotypic cross-reactions among antibodies of the same specificity from different individual rabbits or humans are infrequent (2, 3, 5, 6) and are generally weak when they do occur (7). Eichmann and Kindt (8), however, rece...

Myeloma proteins (1) and antibodies of a given specificity (2, 3) produced in an individual animal possess individually specific or "idiotypic" (4) antigenic determinants. 1 Idiotypic cross-reactions among antibodies of the same specificity from different individual rabbits or humans are infrequent (2, 3, 5, 6) and are generally weak when they do occur (7). Eichmann and Kindt (8), however, rece...

Immunization of rabbits with purified catechol methyltransferase from Candida tropicalis yielded a potent antiserum. Ouchterlony double diffusion analysis showed a single precipitin line. There was no cross reactivity with the catechol methyltransferase from rat and bovine liver. Specific antigen-antibody interaction was demonstrated by a potent inhibitory effect of the antibody on the yeast en...

Antiserum prepared in rabbits against Bacteroides fragilis showed numerous bands when reacted with B. fragilis antigen in Ouchterlony plates. This antiserum also reacted with Bacteroides thetaiotaomicron and showed one band of apparent identity with B. fragilis. The band of identity common for both organisms was lost if the antigen was heated at 80 degrees C for 30 min. Antisera prepared agains...

The Association of Medical Laboratory Immunologists (AMLI) have developed a panel of antinuclear and anticytoplasmic antibody consensus sera that can be useful for enzyme immunoassay (EIA), Ouchterlony, and immunofluorescence assay methods. It was developed to assist in the evaluation of newly available EIA methods for the detection of autoantibodies. The panel of sera was evaluated in several ...

This laboratory has previously isolated a fraction from rat liver nuclei consisting of nuclear pore complexes associated with the proteinaceous lamina which underlies the inner nuclear membrane. Using protein eluted from sodium dodecyl sulfate (SDS) gels, we have prepared antibodies in chickens to each of the three predominant pore complex-lamina bands. Ouchterlony double diffusion analysis sho...