نتایج جستجو برای: orf2 protein

تعداد نتایج: 1234968  

Journal: :Journal of virology 2007
Milan Surjit Shahid Jameel Sunil K Lal

Hepatitis E virus (HEV) is a positive-strand RNA virus that is prevalent in much of the developing world. ORF2 is the major capsid protein of HEV. Although ORF2 is an N-linked glycoprotein, it is abundantly located in the cytoplasm in addition to having membrane and surface localization. The mechanism by which ORF2 protein obtains access to the cytoplasm is unknown. In this report, we prove tha...

Journal: :The Journal of general virology 2002
M B Oleksiewicz A Bøtner P Normann

By selecting phage display libraries with immune sera from experimentally infected pigs, porcine B-cell epitopes in the open reading frame (ORF) 2, 3, 5 and 6 proteins of European-type porcine reproductive and respiratory syndrome virus (PRRSV) were identified. The sequences of all the epitopes were well conserved in European-type PRRSV and even between European- and American-type PRRSV. Accord...

Journal: :The Journal of general virology 2000
P Nawagitgul I Morozov S R Bolin P A Harms S D Sorden P S Paul

Porcine circovirus 2 (PCV2), a single-stranded DNA virus associated with post-weaning multisystemic wasting syndrome of swine, has two potential open reading frames, ORF1 and ORF2, greater than 600 nucleotides in length. ORF1 is predicted to encode a replication-associated protein (Rep) essential for replication of viral DNA, while ORF2 contains a conserved basic amino acid sequence at the N te...

Journal: :Molecular immunology 2008
Huiying Fan Shaobo Xiao Tiezhu Tong Shengpin Wang Lilan Xie Yunbo Jiang Huanchun Chen Liurong Fang

Porcine circovirus type 2 (PCV2) is known to be associated with post-weaning multisystemic wasting syndrome (PMWS), an emerging disease in swine. The development of effective vaccines against PCV2 infection has been accepted as an important strategy in the prophylaxis of PMWS, and a DNA vaccine expressing the major immunogenic capsid (Cap) protein of PCV2 is considered to be a promising candida...

Journal: :Journal of virology 2004
Milan Surjit Shahid Jameel Sunil K Lal

Hepatitis E virus (HEV) is a major human pathogen in much of the developing world. It is a plus-strand RNA virus with a 7.2-kb polyadenylated genome consisting of three open reading frames, ORF1, ORF2, and ORF3. Of these, ORF2 encodes the major capsid protein of the virus and ORF3 encodes a small protein of unknown function. Using the yeast three-hybrid system and traditional biochemical techni...

Journal: :Gene 1994
J Mercado-Blanco J Olivares

An open reading frame, denoted ORF2, present in the replication and stabilization region of plasmid pRmeGR4a of Rhizobium meliloti GR4, was identified by sequence analysis. This 1068-bp ORF2 potentially codes for a 356-amino-acid protein that seems to play a role in pRmeGR4a stabilization. Similarities of the ORF2-encoded protein with eukaryotic cytoskeletal proteins and DNA-binding proteins we...

Journal: :Journal of virology 2004
Porntippa Lekcharoensuk Igor Morozov Prem S Paul Nattarat Thangthumniyom Worawidh Wajjawalku X J Meng

Type 2 porcine circovirus (PCV2) is associated with postweaning multisystemic wasting syndrome in pigs, whereas the genetically related type 1 PCV (PCV1) is nonpathogenic. In this study, seven monoclonal antibodies (MAbs) against PCV2-ORF2 capsid protein were generated, biologically characterized, and subsequently used to map the antigenic sites of PCV2 capsid protein by using infectious PCV DN...

2016
Xinquan Zhang Ivana Bilic Ana Marek Martin Glösmann Michael Hess

The infection of chickens with avian Hepatitis E virus (avian HEV) can be asymptomatic or induces clinical signs characterized by increased mortality and decreased egg production in adult birds. Due to the lack of an efficient cell culture system for avian HEV, the interaction between virus and host cells is still barely understood. In this study, four truncated avian HEV capsid proteins (ORF2-...

Journal: :Journal of clinical microbiology 1993
J He A W Tam P O Yarbough G R Reyes M Carl

The full-length putative structural proteins encoded by open reading frame 2 (ORF2) and ORF3 of hepatitis E virus have been cloned and expressed in recombinant baculovirus. Sera obtained from 28 Sudanese pediatric patients with acute hepatitis and 19 pediatric control patients were analyzed for reactivity to hepatitis E virus by using the baculovirus-expressed ORF2 and ORF3 proteins in a Wester...

2015
Reza Taherkhani Fatemeh Farshadpour Manoochehr Makvandi Hamid Rajabi Memari Ali Reza Samarbafzadeh Nasrin Sharifi Behrouz Naeimi Saeed Tajbakhsh Samad Akbarzadeh

Background. The aim of this study was to evaluate hepatitis E virus (HEV) specific cellular immune responses to truncated ORF2 protein in Iranian patients recovered from HEV infection. Information about HEV-specific immune responses could be useful in finding an effective way for development of HEV vaccine. Methods. A truncated form of HEV ORF2 protein containing amino acids 112-608 was used to...

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